Treponema pallidum Antibody by TP-PA
0050777
Ordering Recommendation
CDC recommended confirmatory test for syphilis. Order if initial screening (eg, RPR, VDRL) is reactive.
Mnemonic
MHA
Methodology
Semi-Quantitative Particle Agglutination
Performed
Mon-Fri
Reported
1-4 days  
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Specimen Required
Patient Preparation
  
Collect
Serum separator tube or plasma separator tube.  
Specimen Preparation
Separate serum or plasma from cells ASAP or within 2 hours of collection. Transfer 1 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 0.1 mL)  
Storage/Transport Temperature
Refrigerated.  
Unacceptable Conditions
CSF or other body fluids.  
Remarks
  
Stability
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)  
Reference Interval
Nonreactive  
Interpretive Data
 
Note
TP-PA is a helpful diagnostic aid for the patient with a reactive reagin test, but presents with atypical signs of primary, secondary, or late syphilis. TP-PA compares favorably with the FTA test, but appears slightly less sensitive in cases of untreated early primary syphilis. In late syphilis, the agreement with FTA is 99%.

VDRL is the preferred test for cerebrospinal fluid. Treponemal tests (TP-PA or FTA) are not recommended for CSF.  FTAs on CSF may be tested, but TP-PA cannot be tested on CSF.
CPT Code(s)
86780
Components
Component Test Code*Component Chart Name
0050777Treponema pallidum Ab by TP-PA
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • MHA-TP
  • Syphilis Antibody by MHA
  • Syphilis TPPA
  • T pallidum TPPA
  • T. pallidum Antibody by MHA
  • TP-PA
  • Treponema Pallidum AB, Particle Agglutination
  • Treponema pallidum Particle Agglutination Assay