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Centromere Antibody, IgG
0050714
Ordering Recommendation

Useful for diagnosis of systemic sclerosis (SSc). Negative results do not rule out SSc. Preferred test is Systemic Scleroderma Comprehensive Panel (2013325).

Mnemonic
ANTICENT
Methodology
Semi-Quantitative Multiplex Bead Assay
Performed
Sun-Sat
Reported
1-2 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
 
Collect
Serum separator tube. 
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.25 mL) 
Storage/Transport Temperature
Refrigerated. 
Unacceptable Conditions
Plasma.  Contaminated, hemolyzed, or severely lipemic specimens. 
Remarks
 
Stability
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles) 
Reference Interval
29 AU/mL or less: Negative
30-40 AU/mL: Equivocal
41 AU/mL or greater: Positive
Interpretive Data
When detected by this multiplex bead assay, the presence of centromere antibodies is mainly associated with CREST syndrome, a variant of systemic sclerosis (SSc). These antibodies target the centromere B, a dominant antigen of the centromeric complex associated with the centromere pattern observed in antinuclear antibody (ANA) testing by IFA. Centromere antibodies may also be seen in a varying percentage of patients with other autoimmune diseases, including diffuse cutaneous SSc, Raynaud syndrome, interstitial pulmonary fibrosis, autoimmune liver disease, systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA).

A negative result indicates no detectable IgG antibodies to centromere B. If the result is negative but clinical suspicion for SSc is strong, consider testing for ANA by IFA along with other antibodies associated with SSc, including Scl-70, U3-RNP, PM/Scl, or Th/To.

Note
CPT Code(s)
83516
Components
Component Test Code*Component Chart NameLOINC
0050714Centromere Ab, IgG29966-9
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • ACA
  • Anti-centromere Antibodies
  • Anti-Centromere Antibody
  • Anticentromere Antibodies
  • Centromere Antibodies, IgG
  • CENTROMERE ANTIBODY
  • Centromere Autoantibodies
  • Centromere B
  • CREST
  • HEp-2