Mycoplasma pneumoniae Antibodies Seroconversion Panel, IgG & IgM
Ordering Recommendation
Semi-Quantitative Enzyme Immunoassay
1-3 days
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Serum separator tube (acute) AND serum separator tube (convalescent).  
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 0.5 mL serum (acute) AND 0.5 mL serum (convalescent) to individual ARUP Standard Transport Tubes. (Min: 0.1 mL each) Mark specimens clearly as "acute" or "convalescent" with appropriate draw dates. If acute specimen was previously tested by ARUP, convalescent specimen must be submitted within 30 days if seroconversion comparison testing is desired.  
Storage/Transport Temperature
Unacceptable Conditions
Contaminated, heat-inactivated, hemolyzed, icteric, or severely lipemic specimens.  
Testing will not be performed unless both acute and convalescent specimens are available.  
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)  
Reference Interval
Test Number Components Reference Interval
0050397Mycoplasma pneumoniae Antibody, IgG < 0.10 U/L: Negative
0.10-​0.32 U/L: Equivocal
> 0.32 U/L: Positive
0050398Mycoplasma pneumoniae Antibody, IgM 0.76 U/L or less: Negative -​ No clinically significant amount of M. pneumoniae IgM antibody detected.
0.77-​0.95 U/L: Low Positive -​ M. pneumoniae-​specific IgM presumptively detected. Collection of a follow-​up sample in one to two weeks is recommended to assure reactivity.
0.96 U/L or greater: Positive -​ Highly significant amount of M. pneumoniae-​specific IgM antibody detected. However, low levels of IgM antibodies may occasionally persist for more than 12 months post-​infection.
Interpretive Data
If both Mycoplasma pneumoniae IgG values are above 0.32 U/L, a ratio will be calculated and the significance will be noted on the report.
CPT Code(s)
86738 x4
Component Test Code*Component Chart Name
0050693M. pneumoniae Antibody, IgM Acute
0050694M. pneumoniae Antibody, IgM Convalescent
0050695M. pneumoniae Antibody, IgG Acute
0050696M. pneumoniae Antibody, IgG Convalescent
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, contact interface support at
Cross References
  • M pneumoniae IgG and IgM seroconversions
  • Mycoplasma G/M Ab Seroconversion