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Extractable Nuclear Antigen Antibodies (RNP, Smith, SSA 52, SSA 60, and SSB)
0050652
Ordering Recommendation

Recommended for the differential diagnosis of SLE and Sjogren syndrome.

Mnemonic
ENA ABS4
Methodology
Semi-Quantitative Multiplex Bead Assay
Performed
Sun-Sat
Reported
1-2 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
 
Collect
Serum separator tube. 
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.3 mL) 
Storage/Transport Temperature
Refrigerated.. 
Unacceptable Conditions
Plasma or other body fluids. Bacterially contaminated or severely lipemic specimens. 
Remarks
 
Stability
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles) 
Reference Interval
Effective May 18, 2015
Test Number
Components
Reference Interval
0050470RNP (U1) (Ribonucleic Protein) (ENA) Antibody, IgG29 AU/mL or less: Negative
30-40 AU/mL: Equivocal
41 AU/mL or greater: Positive
0050085Smith (ENA) Antibody, IgG29 AU/mL or less: Negative
30-40 AU/mL: Equivocal
41 AU/mL or greater: Positive
2012074SSA 52 and 60 (Ro) (ENA) Antibodies, IgG
ComponentsReference Interval
SSA 52 (Ro) (ENA) Antibody, IgG29 AU/mL or less: Negative
30-40 AU/mL: Equivocal
41 AU/mL or greater: Positive
SSA 60 (Ro) (ENA) Antibody, IgG29 AU/mL or less: Negative
30-40 AU/mL: Equivocal
41 AU/mL or greater: Positive

0050692SSB (La) (ENA) Antibody, IgG29 AU/mL or less: Negative
30-40 AU/mL: Equivocal
41 AU/mL or greater: Positive

Interpretive Data
Refer to report.

Note
CPT Code(s)
86235 x5
Components
Component Test Code*Component Chart NameLOINC
0050085Smith (ENA) Antibody, IgG18323-6
0050470Ribonucleic Protein (U1) (ENA) Ab, IgG33983-8
0050691SSA 52 (Ro) (ENA) Antibody, IgG31625-7
0050692SSB (La) (ENA) Antibody, IgG29965-1
2012055SSA 60 (Ro) (ENA) Antibody, IgG53019-6
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Anti-ENA Anti-Ribonuclear Protein
  • Anti-RNP
  • Anti-Sm IgG
  • Anti-U1 RNP
  • Antibodies to Extractable Nuclear Antigens
  • Ribonucleoprotein Autoantibodies
  • RNP (Anti-RNP)
  • RNP (U1) (Ribonucleic Protein) (ENA) Antibody, IgG
  • Ro Ab
  • Scl 70 Antibodies
  • Smith (ENA) Antibody, IgG
  • Smith Antigen
  • Smith Autoantibodies
  • SmRNP Autoantibodies
  • SSA (Ro) (ENA) Antibody, IgG
  • SSA Ro
  • SSB (La) (ENA) Antibody, IgG
  • SSB LA
  • U1 RNP Ab