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Protein Electrophoresis, Serum
0050640
Ordering Recommendation

Detects and quantifies monoclonal protein (M-protein) in serum. Use in screening and monitoring of multiple myeloma and related disorders.

Mnemonic
SPEP
Methodology
Quantitative Capillary Electrophoresis
Performed
Mon-Fri
Reported
1-2 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
 
Collect
Serum separator tube. 
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.5 mL) 
Storage/Transport Temperature
Refrigerated. 
Unacceptable Conditions
Plasma. 
Remarks
 
Stability
After separation from cells: Ambient: 8 hours; Refrigerated: 1 week; Frozen: 1 month 
Reference Interval
Test Number
Components
Reference Interval
 Total Protein-Electrophoresis6.00-8.30 g/dL
 Albumin3.75-5.01 g/dL
 Alpha 1 Globulin0.19-0.46 g/dL
 Alpha 2 Globulin0.48-1.05 g/dL
 Beta Globulin0.48-1.10 g/dL
 Gamma0.62-1.51 g/dL

Interpretive Data
Serum protein electrophoresis, when used as a screening test, is useful in the detection of various pathophysiologic states such as inflammation, protein loss, gammopathies, and other dysproteinemias. However, immunofixation electrophoresis (IFE) is a more sensitive technique for the identification of small M-proteins found in patients with amyloidosis, early or treated myeloma or macroglobulinemia, solitary plasmacytoma, or extramedullary plasmacytoma.

Note
A copy of the graph will follow final report.
For CSF electrophoresis, refer to Protein Electrophoresis, CSF (ARUP test code 0050590).
CPT Code(s)
84160; 84165
Components
Component Test Code*Component Chart NameLOINC
0050020Albumin2862-1
0050035Alpha 1 Globulin2865-4
0050060Alpha 2 Globulin2868-8
0050110Beta Globulin2871-2
0050270Gamma2874-6
0050545Total Protein-Electrophoresis2885-2
2005168SPEP/IFE Interpretation49275-1
2012453EER Protein Electrophoresis, Serum
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Protein ELP
  • Serum Protein Electrophoresis
  • SPEP