Protein Electrophoresis, Serum
0050640
Ordering Recommendation
Detects and quantifies monoclonal protein (M-protein) in serum. Use in screening and monitoring of multiple myeloma and related disorders.
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Mnemonic
SPEP
Methodology
Quantitative Capillary Electrophoresis
Performed
Mon-Fri
Reported
1-2 days
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
 
Collect
Serum separator tube.  
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.5 mL)  
Storage/Transport Temperature
Refrigerated.  
Unacceptable Conditions
Plasma.  
Remarks
 
Stability
After separation from cells: Ambient: 8 hours; Refrigerated: 1 week; Frozen: 1 month  
Reference Interval
Test Number Components Reference Interval
Total Protein-​Electrophoresis, Serum 6.00-​8.30 g/dL
Albumin 3.75-​5.01 g/dL
Alpha-​1 Globulins 0.19-​0.46 g/dL
Alpha-​2 Globulins 0.48-​1.05 g/dL
Beta Globulins 0.48-​1.10 g/dL
Gamma 0.62-​1.51 g/dL
Interpretive Data
Serum protein electrophoresis, when used as a screening test, is useful in the detection of various pathophysiologic states such as inflammation, protein loss, gammopathies, and other dysproteinemias. However, immunofixation electrophoresis (IFE) is a more sensitive technique for the identification of small M-proteins found in patients with amyloidosis, early or treated myeloma or macroglobulinemia, solitary plasmacytoma, or extramedullary plasmacytoma.
Note
A copy of the graph will follow final report.
For CSF electrophoresis, refer to Protein Electrophoresis, CSF (ARUP test code 0050590).
CPT Code(s)
84160; 84165
Components
Component Test Code*Component Chart Name
0050020Albumin
0050035Alpha 1 Globulin
0050060Alpha 2 Globulin
0050110Beta Globulin
0050270Gamma
0050545Total Protein-Electrophoresis
2005168SPEP/IFE Interpretation
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Cross References
  • Protein ELP
  • Serum Protein Electrophoresis
  • SPEP