Protein Electrophoresis, Serum
0050640
Ordering Recommendation
Detects and quantifies monoclonal protein (M-protein) in serum. Use in screening and monitoring of multiple myeloma and related disorders.
Mnemonic
SPEP
Methodology
Quantitative Capillary Electrophoresis
Performed
Mon-Fri
Reported
1-2 days  
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Specimen Required
Patient Preparation
  
Collect
Serum separator tube.  
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.5 mL)  
Storage/Transport Temperature
Refrigerated.  
Unacceptable Conditions
Plasma.  
Remarks
   
Stability
After separation from cells: Ambient: 8 hours; Refrigerated: 1 week; Frozen: 1 month  
Reference Interval
 
 
Test Number Components Reference Interval
 Total Protein-​Electrophoresis, Serum 6.00-​8.30 g/dL
 Albumin 3.75-​5.01 g/dL
 Alpha-​1 Globulins 0.19-​0.46 g/dL
 Alpha-​2 Globulins 0.48-​1.05 g/dL
 Beta Globulins 0.48-​1.10 g/dL
 Gamma  0.62-​1.51 g/dL
Interpretive Data
Serum protein electrophoresis, when used as a screening test, is useful in the detection of various pathophysiologic states such as inflammation, protein loss, gammopathies, and other dysproteinemias. However, immunofixation electrophoresis (IFE) is a more sensitive technique for the identification of small M-proteins found in patients with amyloidosis, early or treated myeloma or macroglobulinemia, solitary plasmacytoma, or extramedullary plasmacytoma.  
Note
A copy of the graph will follow final report.
For CSF electrophoresis, refer to Protein Electrophoresis, CSF (ARUP test code 0050590).
CPT Code(s)
84160; 84165
Components
Component Test Code*Component Chart Name
0050020Albumin
0050035Alpha 1 Globulin
0050060Alpha 2 Globulin
0050110Beta Globulin
0050270Gamma
0050545Total Protein-Electrophoresis
2005168SPEP/IFE Interpretation
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Aliases
  • Protein ELP
  • Serum Protein Electrophoresis
  • SPEP