Kappa and Lambda Free Light Chains (Bence Jones Protein), Quantitative, Urine
Ordering Recommendation

Aids in the diagnosis and management of multiple myeloma and related disorders. Preferred test to detect and quantify urinary free light chains (Bence Jones protein).

Qualitative Immunofixation Electrophoresis/Quantitative Nephelometry
1-5 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
24-hour urine. Refrigerate during collection. Also acceptable: Random urine specimens and urine supernate.  
Specimen Preparation
Transfer two 4 mL aliquots from a well-mixed 24-hour collection to individual ARUP Standard Transport Tubes. (Min: 3 mL) Record total volume and collection time interval on transport tube and test request form.    
Storage/Transport Temperature
Unacceptable Conditions
Ambient: 2 hours; Refrigerated: 1 week; Frozen: Unacceptable  
Reference Interval
Test Number
Reference Interval
 Total Protein10-140 mg/d
 Albumin, UrineDetected
 Alpha-1, UrineNone detected
 Alpha-2, UrineNone detected
 Urine Beta GlobulinNone detected
 Gamma, UrineNone detected
 Free Urinary Kappa Light Chains0.14 - 2.42 mg/dL
 Free Urinary Kappa Excretion/DayBy report
 Free Urinary Lambda Light Chain0.02 - 0.67 mg/dL
 Free Urinary Lambda Excretion/DayBy report
 Free Urinary Kappa/Lambda Ratio2.04 - 10.37 (ratio)
 IFE InterpretationBy report

Interpretive Data
Total urinary protein is determined nephelometrically by adding the albumin and kappa and/or lambda light chains. This value may not agree with the total protein as determined by chemical methods, which characteristically underestimate urinary light chains.

CPT Code(s)
84156; 86335; 83883 x2
Component Test Code*Component Chart NameLOINC
0050025Albumin, Urine29946-1
0050050Alpha-1, Urine29945-3
0050055Alpha-2, Urine29947-9
0050120Urine Beta Globulin53772-0
0050285Gamma, Urine53773-8
0050565Total Protein2889-4
0050683Free Urinary Kappa Light Chains38176-4
0050684Free Urinary Kappa Excretion/Day38177-2
0050685Free Urinary Lambda Light Chain38178-0
0050686Free Urinary Lambda Excretion/Day38169-9
0050687Free Urinary Kappa/Lambda Ratio41759-2
0050688IFE Interpretation13440-3
0097110Total Volume19153-6
0097111Hours Collected30211-7
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • Free Kappa & Lambda Light Chains, IFE
  • Free Urinary Kappa Light Chains
  • Free Urinary Kappa/Lambda Ratio
  • Free Urinary Lambda Light Chain
  • IFE urine, Immunofixation Electrophoresis, urine
  • Monoclonal protein detection and quantitation, urine
  • Monoclonal Protein Study, Random Urine
  • UPEP
  • Urinary Light Chains, IFE