Hemoglobin (Hb) A2 and F by Column
Ordering Recommendation
Quantifies HbA2 and HbF in whole blood. Aids in the management of sickle cell disease and in the identification of beta thalassemia carriers.
High Performance Liquid Chromatography
1-4 days  
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Specimen Required
Patient Preparation
Lavender (EDTA) or pink (K2EDTA).  
Specimen Preparation
Transport 5 mL whole blood. (Min: 0.2 mL)  
Storage/Transport Temperature
Unacceptable Conditions
Frozen or room temperature specimens.  
Ambient: Unacceptable; Refrigerated: 1 week; Frozen: Unacceptable  
Reference Interval
Effective August 19, 2013
Age-​Definied Normal Hemoglobin Reference Intervals
Age Hb A2 Percent Hb F Percent
0-​1 month 0.0-​1.4 45.8-​91.7
2 months 0.0-​2.0 32.7-​85.2
3 months 0.1-​2.6 14.5-​73.7
4 months 0.8-​3.0 4.2-​56.9
5 months 1.5-​3.3 1.0-​38.1
6-​8 months 1.8-​3.5 0.9-​19.4
9-​12 months 1.9-​3.5 0.6-​11.6
13-​23 months 1.9-​3.5 0.0-​8.5
2 years and older 2.0-​3.5 0.0-​2.1
Interpretive Data
In laboratorytesting to confirm a diagnosis of a β-thalassemia trait diagnosis, HbA2 levels should be considered in conjunction with family historyand additional laboratory data, including serum iron and iron binding capacity, red cell morphology, hemoglobin, hematocrit, and mean corpuscular volume (MCV).

Patients with a combination of iron deficiency and β-thalassemia may have a normal A2level. In these cases, elevated A2level cannot be used to screen for β-thalassemia in these cases.
Patient State Hb A2 Level Hb F Level
Heterozygous β-​thalassemia 4-​9% 1-​5%
Homozygous β-​thalassemia Normal or Increased 80-​100%
Heterozygous HPFH Less than 1.5% 10-​20%
Homozygous HPFH Absent 100%
Recommend quantitation of hemoglobin for definitive diagnosis after 1 year of age.
CPT Code(s)
Component Test Code*Component Chart NameLOINC
0050300Hemoglobin A24551-8
0050315Hemoglobin F4576-5
0050614Hemoglobin A2 and F Interpretation21026-0
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • A2 Hemoglobin
  • F-Hemoglobin
  • Hemoglbinopathy (Hb) A2
  • Hemoglobin A2
  • Hemoglobin A2 and F, Blood
  • Hemoglobin F