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Hemoglobin (Hb) A2 and F by Column
0050613
Ordering Recommendation

Quantifies HbA2 and HbF in whole blood. Aids in the management of sickle cell disease and in the identification of beta thalassemia carriers.

Mnemonic
HGBA2F COL
Methodology
High Performance Liquid Chromatography
Performed
Sun-Sat
Reported
1-4 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
 
Collect
Lavender (EDTA) or pink (K2EDTA). 
Specimen Preparation
Transport 5 mL whole blood. (Min: 0.2 mL) 
Storage/Transport Temperature
Refrigerated. 
Unacceptable Conditions
Frozen or room temperature specimens. 
Remarks
 
Stability
Ambient: Unacceptable; Refrigerated: 1 week; Frozen: Unacceptable 
Reference Interval
Effective August 19, 2013
Age-Definied Normal Hemoglobin Reference Intervals
AgeHb A2 PercentHb F Percent
0-1 month0.0-1.445.8-91.7
2 months0.0-2.032.7-85.2
3 months0.1-2.614.5-73.7
4 months0.8-3.04.2-56.9
5 months1.5-3.31.0-38.1
6-8 months1.8-3.50.9-19.4
9-12 months1.9-3.50.6-11.6
13-23 months1.9-3.50.0-8.5
2 years and older2.0-3.50.0-2.1

Interpretive Data
In laboratorytesting to confirm a diagnosis of a β-thalassemia trait diagnosis, HbA2 levels should be considered in conjunction with family historyand additional laboratory data, including serum iron and iron binding capacity, red cell morphology, hemoglobin, hematocrit, and mean corpuscular volume (MCV).

Patients with a combination of iron deficiency and β-thalassemia may have a normal A2level. In these cases, elevated A2level cannot be used to screen for β-thalassemia in these cases.

Patient State
Hb A2 Level
Hb F Level
Heterozygous β-thalassemia4-9%1-5%
Homozygous β-thalassemiaNormal or Increased80-100%
Heterozygous HPFHLess than 1.5%10-20%
Homozygous HPFHAbsent100%

Note
Recommend quantitation of hemoglobin for definitive diagnosis after 1 year of age.
CPT Code(s)
83021
Components
Component Test Code*Component Chart NameLOINC
0050300Hemoglobin A24551-8
0050315Hemoglobin F4576-5
0050614Hemoglobin A2 and F Interpretation21026-0
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • A2 Hemoglobin
  • F-Hemoglobin
  • Hemoglbinopathy (Hb) A2
  • Hemoglobin A2
  • Hemoglobin A2 and F, Blood
  • Hemoglobin F