Hemoglobin (Hb) A2 and F by Column
0050613
Ordering Recommendation
Quantifies HbA2 and HbF in whole blood. Aids in the management of sickle cell disease and in the identification of beta thalassemia carriers.
Submit With Order
Mnemonic
HGBA2F COL
Methodology
High Performance Liquid Chromatography
Performed
Sun-Sat
Reported
1-4 days
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
 
Collect
Lavender (EDTA) or pink (K2EDTA).  
Specimen Preparation
Transport 5 mL whole blood. (Min: 0.2 mL)  
Storage/Transport Temperature
Refrigerated.  
Unacceptable Conditions
Frozen or room temperature specimens.  
Remarks
 
Stability
Ambient: Unacceptable; Refrigerated: 1 week; Frozen: Unacceptable  
Reference Interval
Effective August 19, 2013
Age-​Definied Normal Hemoglobin Reference Intervals
Age Hb A2 Percent Hb F Percent
0-​1 month 0.0-​1.4 45.8-​91.7
2 months 0.0-​2.0 32.7-​85.2
3 months 0.1-​2.6 14.5-​73.7
4 months 0.8-​3.0 4.2-​56.9
5 months 1.5-​3.3 1.0-​38.1
6-​8 months 1.8-​3.5 0.9-​19.4
9-​12 months 1.9-​3.5 0.6-​11.6
13-​23 months 1.9-​3.5 0.0-​8.5
2 years and older 2.0-​3.5 0.0-​2.1
Interpretive Data
In laboratory testing to confirm a diagnosis of a β-thalassemia trait diagnosis, Hb A2 levels should be considered in conjunction with family history and additional laboratory data, including serum iron and iron binding capacity, red cell morphology, hemoglobin, hematocrit, and mean corpuscular volume (MCV).

Patients with a combination of iron deficiency and β-thalassemia may have a normal A2 level. In these cases, elevated A2 level cannot be used to screen for β-thalassemia in these cases.
Patient State Hb A2 Level Hb F Level
Heterozygous β-​thalassemia 4-​9% 1-​5%
Homozygous β-​thalassemia Normal or Increased 80-​100%
Heterozygous HPFH Less than 1.5% 10-​20%
Homozygous HPFH Absent 100%
Note
Recommend quantitation of hemoglobin for definitive diagnosis after 1 year of age.
CPT Code(s)
83021
Components
Component Test Code*Component Chart Name
0050300Hemoglobin A2
0050315Hemoglobin F
0050614Hemoglobin A2 and F Interpretation
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Cross References
  • A2 Hemoglobin
  • F-Hemoglobin
  • Hemoglbinopathy (Hb) A2
  • Hemoglobin A2
  • Hemoglobin A2 and F, Blood
  • Hemoglobin F