Hemoglobin Evaluation with Reflex to Electrophoresis and/or RBC Solubility
0050610
Ordering Recommendation
Effective test for screening and follow-up of individuals with known hemoglobinopathies. The optimal test for the initial diagnosis of a suspected hemoglobinopathy is the Hemoglobin Evaluation Reflexive Cascade (2005792).
Mnemonic
HGBEL
Methodology
High Performance Liquid Chromatography/Electrophoresis/RBC Solubility
Performed
Sun-Sat
Reported
1-7 days
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
 
Collect
Lavender (EDTA) or pink (K2EDTA).  
Specimen Preparation
Transport 5 mL whole blood. (Min: 0.2 mL)  
Storage/Transport Temperature
Refrigerated.  
Unacceptable Conditions
Frozen or room temperature specimens.  
Remarks
 
Stability
Ambient: Unacceptable; Refrigerated: 1 week; Frozen: Unacceptable  
Reference Interval
Effective August 19, 2013
Age-​Defined Normal Hemoglobin Reference Intervals
Age Hb A Percent Hb A2 Percent Hb F Percent Hb S Percent Hb C Percent Hb E Percent Hb Other Percent
0-​1 month
2 months
3 months
4 months
5 months
6-​8 months
9-​12 months
13-​23 months
2 years and older
7.6-​54.8
14.7-​70.1
26.6-​81.8
43.0-​89.5
60.8-​94.0
78.2-​96.6
86.1-​97.2
85.1-​97.7
95.0-​97.9
0.0-​1.4
0.0-​2.0
0.1-​2.6
0.8-​3.0
1.5-​3.3
1.8-​3.5
1.9-​3.5
1.9-​3.5
2.0-​3.5
45.8-​91.7
32.7-​85.2
14.5-​73.7
4.2-​56.9
1.0-​38.1
0.9-​19.4
0.6-​11.6
0.0-​8.5
0.0-​2.1
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
Interpretive Data
Note
If HPLC detects any abnormal peaks suggestive of a hemoglobin variant, then further testing (RBC Solubility, Acid or Alkaline Electrophoresis, Capillary Electrophoresis) will be added to aid in identification and confirmation. Additional charges apply.

In infants age 1 year and older, quantitation of hemoglobin is recommended for definitive diagnosis. Abnormal hemoglobin variants may require additional testing, up to 10 days.
CPT Code(s)
83021; if reflexed, add 85660 or 83020 or 83020 or 83021
Components
Component Test Code*Component Chart Name
0050295Hemoglobin A
0050300Hemoglobin A2
0050305Hemoglobin C
0050310Hemoglobin E
0050315Hemoglobin F
0050320Hemoglobin - Other
0050325Hemoglobin S
0050611Hemoglobin Evaluation
0081268Hemoglobin, Acid Electrophoresis
0081269Hemoglobin, Alkaline Electrophoresis
0081270Sickle Cell Solubility
2008793Hemoglobin, Capillary Electrophoresis
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Cross References
  • Evaluation, Hemoglobin
  • Fetal Hemoglobin (Hemoglobin F)
  • Hb
  • Hb ELP
  • Hb IEF
  • Hemoglobin Evaluation
  • Hemoglobin Fractionation
  • Hemoglobinopathy Fractionation Profile
  • Hgb
  • Sickle Cell Anemia Screen
  • Sickle Cell Disease
  • Sickle Cell Screen