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Hemoglobin Evaluation with Reflex to Electrophoresis and/or RBC Solubility
0050610
Ordering Recommendation
Effective test for screening and follow-up of individuals with known hemoglobinopathies. The optimal test for the initial diagnosis of a suspected hemoglobinopathy is the Hemoglobin Evaluation Reflexive Cascade (2005792).
Mnemonic
HGBEL
Methodology
High Performance Liquid Chromatography/Electrophoresis/RBC Solubility
Performed
Sun-Sat
Reported
1-5 days
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
 
Collect
Lavender (EDTA) or pink (K2EDTA).  
Specimen Preparation
Transport 5 mL whole blood. (Min: 0.2 mL)  
Storage/Transport Temperature
Refrigerated.  
Unacceptable Conditions
Frozen or room temperature specimens.  
Remarks
 
Stability
Ambient: Unacceptable; Refrigerated: 1 week; Frozen: Unacceptable  
Reference Interval
Effective August 19, 2013
Age-Defined Normal Hemoglobin Reference Intervals
Age
Hb A Percent
Hb A2 Percent
Hb F Percent
Hb S Percent
Hb C Percent
Hb E Percent
Hb Other Percent
0-1 month
2 months
3 months
4 months
5 months
6-8 months
9-12 months
13-23 months
2 years and older
7.6-54.8
14.7-70.1
26.6-81.8
43.0-89.5
60.8-94.0
78.2-96.6
86.1-97.2
85.1-97.7
95.0-97.9
0.0-1.4
0.0-2.0
0.1-2.6
0.8-3.0
1.5-3.3
1.8-3.5
1.9-3.5
1.9-3.5
2.0-3.5
45.8-91.7
32.7-85.2
14.5-73.7
4.2-56.9
1.0-38.1
0.9-19.4
0.6-11.6
0.0-8.5
0.0-2.1
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0

Interpretive Data


Note
If abnormal peaks suggestive of a hemoglobin variant are detected, then RBC Solubility and/or Capillary
Electrophoresis will be added to aid in confirmation and identification of the variant. Additional charges apply.
Quantitation of hemoglobin is recommended for a definitive diagnosis in infants 1 year and older.

Additional testing of detected hemoglobin variants may increase turnaround time up to 10 days.
CPT Code(s)
83021; if reflexed add 85660 and/or 83020
Components
Component Test Code*Component Chart NameLOINC
0050295Hemoglobin A4547-6
0050300Hemoglobin A24551-8
0050305Hemoglobin C4563-3
0050310Hemoglobin E4575-7
0050315Hemoglobin F4576-5
0050320Hemoglobin - Other48343-8
0050325Hemoglobin S4625-0
0050611Hemoglobin Evaluation21026-0
0081270Sickle Cell Solubility6864-3
2008793Hemoglobin, Capillary Electrophoresis13514-5
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Evaluation, Hemoglobin
  • Fetal Hemoglobin (Hemoglobin F)
  • Hb
  • Hb ELP
  • Hb IEF
  • Hemoglobin Evaluation
  • Hemoglobin Fractionation
  • Hemoglobinopathy Fractionation Profile
  • Hgb
  • Sickle Cell Anemia Screen
  • Sickle Cell Disease
  • Sickle Cell Screen