Diphtheria & Tetanus Antibodies, IgG
0050595
Ordering Recommendation
Determination of antibody status pre- and/or post-vaccination.
Submit With Order
Mnemonic
D/T
Methodology
Quantitative Multiplex Bead Assay
Performed
Sun-Sat
Reported
1-2 days
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
 
Collect
Serum separator tube. "Pre" and "post" vaccination specimens should be submitted for testing. "Post" specimen should be drawn 30 days after immunization.  
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.15 mL) Mark specimens clearly as "Pre-Vaccine" or "Post-Vaccine". If shipped separately, "Post" specimen must be received within 60 days of "Pre" specimen.  
Storage/Transport Temperature
Refrigerated  
Unacceptable Conditions
Plasma or other body fluids.  
Remarks
 
Stability
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)  
Reference Interval
Antibody concentrations of > 0.1 IU/mL are usually considered protective for diphtheria or tetanus.
Interpretive Data
Responder status is determined according to the ratio of a one-month post-vaccination sample to pre-vaccination concentration of IgG antibodies as follows:

Diphtheria and tetanus:
1. If the post-vaccination concentration is less than 1.0 IU/mL, the patient is considered a nonresponder.
2. If the post-vaccination concentration is greater than or equal to 1.0 IU/mL, a patient with a ratio of less than 1.5 is a nonresponder, a ratio of 1.5 to less than 3.0, is a weak responder, and a ratio of 3.0 or greater, is a good responder.
3. If the pre-vaccination concentration is greater than 1.0 IU/mL, it may be difficult to assess the response based on a ratio alone. A post-vaccination concentration above 2.5 IU in this case is adequate.

See Compliance Statement B: www.aruplab.com/CS
Note
CPT Code(s)
86317 x2
Components
Component Test Code*Component Chart Name
0050210Diphtheria Antibody, IgG
0050535Tetanus Antibody, IgG
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Cross References
  • C. tetani
  • Clostridium tetani
  • Corynebacterium diphtheriae Antitoxin
  • Diphtheria Antitoxoid
  • Immune Response
  • nti-tetanus toxoid
  • Tetanus Immune Response
  • Tetanus toxoid antibodies
  • Tetanus/Diphtheria Antibody Panel
  • Tetanus/Diptheria Vaccine Response
  • Vaccine Response