Evaluate the ability of a patient to produce antibody to a protein conjugated bacterial (H. influenza) vaccine to rule out antibody deficiency.
- Patient Preparation
- Serum separator tube. "Pre" and 30-day "post" Haemophilus influenzae b vaccination specimens should be submitted together for testing. "Post" specimen should be drawn 30 days after immunization and must be received within 60 days of "pre" specimen.
- Specimen Preparation
- Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.15 mL) Mark specimens clearly as "Pre-Vaccine" or "Post-Vaccine".
- Storage/Transport Temperature
- Unacceptable Conditions
- Plasma or other body fluids. Contaminated, hemolyzed or, severely lipemic specimens.
- After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)
≥ 1.0 µg/mL = Antibody to H. influenzae b detected. Suggestive of protection.
1. If the post-vaccination concentration is less than 3.0 µg/mL, the patient is considered to be a nonresponder.
2. If the post-vaccination concentration is greater than or equal to 3.0 µg/mL, a patient with a ratio of greater than or equal to 4 is a good responder, a ratio of 2-4 is a weak responder, and a ratio of less than 2 is considered a nonresponder.
|Component Test Code*||Component Chart Name||LOINC|
|0050542||Haemophilus influenzae b Antibody, IgG||11256-5|
- H. influenzae Antibody
- Haemophilus influenzae b Vaccine Response
- HIB (Haemophilus influenzae Type B) Vaccine Response
- Vaccine Response