Rapid Plasma Reagin (RPR) with Reflex to Titer and TP-PA Confirmation
Ordering Recommendation
CDC recommended test for the screening and diagnosis of syphilis. For RPR test that follows reverse algorithm, refer to Rapid Plasma Reagin (RPR) with Reflex to RPR Titer or T. pallidum Antibody by Particle  Agglutination  (2007443).
Semi-Quantitative Charcoal Agglutination/Semi-Quantitative Particle Agglutination
1-4 days  
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Specimen Required
Patient Preparation
Serum separator tube.  
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.25 mL) Do not freeze. Also acceptable: Plasma, if testing is completed within 48 hours of collection.  
Storage/Transport Temperature
Unacceptable Conditions
CSF or other body fluids.  
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)  
Reference Interval
Test Number Components Reference Interval
 Rapid Plasma Reagin (RPR) Effective May 21, 2012
RPR (+) = Reactive
RPR (-​) = Nonreactive
 Rapid Plasma Reagin (RPR) Titer < 1:1
 Treponema pallidum Antibody by TP-​PA Nonreactive
Interpretive Data
This panel is for clients in states where automatic confirmation using a treponemal test is required for all reactive RPR tests.

If RPR is reactive, then a titer to endpoint and TP-PA confirmation will be added. Additional charges apply.
CPT Code(s)
86592; if reflexed, add 86593 and 86780
Component Test Code*Component Chart NameLOINC
0050472Rapid Plasma Reagin (RPR)20507-0
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • Rapid Plasma Reagin (RPR) with MHA-TP Confirmation
  • RPR (Diagnosis) with Reflex to Titer and Confirmatory Testing
  • RPR Reflex Titer & TPPA
  • RPR Reflex TPPA & Titer
  • RPR Reflex with confirmation
  • Treponema pallidum with Reflex to Titer and TP-PA Confirmation