Herpes Simplex Virus Types 1 and 2 Glycoprotein G-Specific Antibodies, IgG by Immunoblot
Ordering Recommendation
Qualitative Immunoblot
1-8 days  
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Specimen Required
Patient Preparation
Serum separator tube.  
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.1 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of acute specimens. Mark specimens plainly as "acute" or "convalescent."  
Storage/Transport Temperature
Unacceptable Conditions
Contaminated, heat-inactivated, hemolyzed, or hyperlipemic specimens.  
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)  
Reference Interval
By report  
Interpretive Data
Individuals infected with HSV may not exhibit detectable IgG antibody to type specific HSV antigens 1 and 2 in the early stages of infection. Detection of antibody presence in these cases may only be possible using a nontype-specific screening test. All results from this and other serologies must be correlated with clinical history, epidemiological data, and other data available to the attending physician in making the differentiation between HSV type 1 and HSV type 2.
Patient Interpretation Band Reactivity
 HSV Common Antigen HSV Glycoprotein-​1 HSV Glycoprotein-​2
HSV-​1 positive.  Presumptive evidence of HSV-​1 exposure. + + Neg
HSV-​2 positive.  Presumptive evidence of HSV-​2 exposure + Neg +
HSV-​1 and HSV-​2 positive.  Presumptive evidence of HSV-​1 and HSV-​2 exposure. + + +
HSV equivocal. A second sample should be drawn in two to four weeks and retested by Immunoblot or other type-​specific HSV IgG method. + Neg Neg
Negative. No evidence of HSV exposure detected. Neg Neg/+ Neg/+
CPT Code(s)
86695; 86696
Component Test Code*Component Chart NameLOINC
0050456HSV 1 Antibody IgG Immunoblot17850-9
0050457HSV 2 Antibody IgG Immunoblot16955-7
0050458HSV Anti-Common Antigen29957-8
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • HSV
  • HSV immunoblot
  • HSV Type Specific 1 & 2
  • HSV typing 1 and 2 glycoprotein G