Herpes Simplex Virus Types 1 and 2 Glycoprotein G-Specific Antibodies, IgG by Immunoblot
New York DOH Approval Status
This test is New York DOH approved.
- Patient Preparation
- Serum separator tube.
- Specimen Preparation
- Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.1 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of acute specimens. Mark specimens plainly as "acute" or "convalescent."
- Storage/Transport Temperature
- Unacceptable Conditions
- Contaminated, heat-inactivated, hemolyzed, or hyperlipemic specimens.
- After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)
Individuals infected with HSV may not exhibit detectable IgG antibody to type specific HSV antigens 1 and 2 in the early stages of infection. Detection of antibody presence in these cases may only be possible using a nontype-specific screening test. All results from this and other serologies must be correlated with clinical history, epidemiological data, and other data available to the attending physician in making the differentiation between HSV type 1 and HSV type 2.
|Patient Interpretation||Band Reactivity|
|HSV Common Antigen||HSV Glycoprotein-1||HSV Glycoprotein-2|
|HSV-1 positive. Presumptive evidence of HSV-1 exposure.||+||+||Neg|
|HSV-2 positive. Presumptive evidence of HSV-2 exposure||+||Neg||+|
|HSV-1 and HSV-2 positive. Presumptive evidence of HSV-1 and HSV-2 exposure.||+||+||+|
|HSV equivocal. A second sample should be drawn in two to four weeks and retested by Immunoblot or other type-specific HSV IgG method.||+||Neg||Neg|
|Negative. No evidence of HSV exposure detected.||Neg||Neg/+||Neg/+|
Type-specific ELISA antibody tests for herpes simplex virus types 1 and 2 antibody, IgG are now available which use the same cloned antigens as in the immunoblot. Sensitivity and specificity for both the type-specific ELISA and immunoblot are similar. The ELISA assay is offered Sunday through Saturday. Refer to Herpes Simplex Virus Type 1 Glycoprotein G-Specific Antibody, IgG by ELISA (HerpeSelect®) (0050292) and Herpes Simplex Virus Type 2 Glycoprotein G-Specific Antibody, IgG by ELISA (HerpeSelect®) (0050294).
|Component Test Code*||Component Chart Name|
|0050456||HSV 1 Antibody IgG Immunoblot|
|0050457||HSV 2 Antibody IgG Immunoblot|
|0050458||HSV Anti-Common Antigen|
- HerpeSelect (Herpes Simplex Virus Type 1 & Type 2 Glycoprotein G-Specific Antibodies, IgG by Immunoglot
- HSV (Herpes Simplex Virus Type 1 & Type 2 Glycoprotein G-Specific Antibodies, IgG by Immunoblot)
- HSV Type Specific 1 & 2 (Herpes Simplex Virus Type 1 & Type 2 Glycoprotein G-Specific Antibodies, IgG by Immunoblot