Herpes Simplex Virus Types 1 and 2 Glycoprotein G-Specific Antibodies, IgG by Immunoblot
0050459
Ordering Recommendation
 
Mnemonic
HSV BLOT
Methodology
Qualitative Immunoblot
Performed
Mon
Reported
1-8 days  
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Specimen Required
Patient Preparation
  
Collect
Serum separator tube.  
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.1 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of acute specimens. Mark specimens plainly as "acute" or "convalescent."  
Storage/Transport Temperature
Refrigerated.  
Unacceptable Conditions
Contaminated, heat-inactivated, hemolyzed, or hyperlipemic specimens.  
Remarks
  
Stability
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)  
Reference Interval
By report  
Interpretive Data
Individuals infected with HSV may not exhibit detectable IgG antibody to type specific HSV antigens 1 and 2 in the early stages of infection. Detection of antibody presence in these cases may only be possible using a nontype-specific screening test. All results from this and other serologies must be correlated with clinical history, epidemiological data, and other data available to the attending physician in making the differentiation between HSV type 1 and HSV type 2.
 
Patient Interpretation Band Reactivity
 HSV Common Antigen HSV Glycoprotein-​1 HSV Glycoprotein-​2
HSV-​1 positive.  Presumptive evidence of HSV-​1 exposure. + + Neg
HSV-​2 positive.  Presumptive evidence of HSV-​2 exposure + Neg +
HSV-​1 and HSV-​2 positive.  Presumptive evidence of HSV-​1 and HSV-​2 exposure. + + +
HSV equivocal. A second sample should be drawn in two to four weeks and retested by Immunoblot or other type-​specific HSV IgG method. + Neg Neg
Negative. No evidence of HSV exposure detected. Neg Neg/+ Neg/+
Note
Type-specific ELISA antibody tests for herpes simplex virus types 1 and 2 antibody, IgG are now available which use the same cloned antigens as in the immunoblot.  Sensitivity and specificity for both the type-specific ELISA and immunoblot are similar.  The ELISA assay is offered Sunday through Saturday.  Refer to Herpes Simplex Virus Type 1 Glycoprotein G-Specific Antibody, IgG by ELISA (HerpeSelect®) (0050292) and Herpes Simplex Virus Type 2 Glycoprotein G-Specific Antibody, IgG by ELISA (HerpeSelect®) (0050294).
CPT Code(s)
86695; 86696
Components
Component Test Code*Component Chart Name
0050456HSV 1 Antibody IgG Immunoblot
0050457HSV 2 Antibody IgG Immunoblot
0050458HSV Anti-Common Antigen
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Aliases
  • HSV
  • HSV immunoblot
  • HSV Type Specific 1 & 2
  • HSV typing 1 and 2 glycoprotein G