Herpes Simplex Virus Type 1 and/or 2 Antibodies, IgM by ELISA, CSF
Ordering Recommendation
Semi-Quantitative Enzyme-Linked Immunosorbent Assay
Within 24 hours  
New York DOH Approval Status
Specimens from New York clients will be sent out to a New York DOH approved laboratory, if possible.
Submit With Order
Specimen Required
Patient Preparation
Specimen Preparation
Transfer 0.5 mL CSF to an ARUP Standard Transport Tube. (Min: 0.2 mL)  
Storage/Transport Temperature
Unacceptable Conditions
Specimen types other than CSF. Contaminated or heat-inactivated specimens.  
Indicate source on test request form.  
Ambient: 8 hours; Refrigerated: 2 weeks; Frozen: 1 year  
Reference Interval
0.89 IV or less: Negative - No significant level of detectable HSV IgM antibody.
0.90-1.09 IV: Equivocal - Questionable presence of IgM antibodies.  Repeat testing in 10-14 days may be helpful.
1.10 IV or greater: Positive - IgM antibody to HSV detected, which may indicate a current or recent infection. However, low levels of IgM antibodies may occasionally persist for more than 12 months post-infection.  
Interpretive Data
The detection of antibodies to herpes simplex virus in CSF may indicate central nervous system infection. However, consideration must be given to possible contamination by blood or transfer or serum antibodies across the blood-brain barrier.

Fourfold or greater rise in CSF antibodies to herpes on specimens at least 4 weeks apart are found in 74-94% of patients with herpes encephalitis. Specificity of the test based on a single CSF testing is not established. Presently PCR is the primary means of establishing a diagnosis of herpes encephalitis.

Compliance Statement B: This test was developed and its performance characteristics determined by ARUP Laboratories. The U.S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.
CPT Code(s)
Component Test Code*Component Chart NameLOINC
0050408HSV 1 and/or 2 Antibodies IgM, CSF63432-9
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • HSV
  • HSV antibodies
  • HSV IgM and IgG