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C1q Binding Assay
0050301
Ordering Recommendation

Detect circulating immune complexes. Consider ordering Immune Complex Panel (0050667), which contains both Raji cell immune complex and C1q binding assays.

Mnemonic
C1Q
Methodology
Semi-Quantitative Enzyme-Linked Immunosorbent Assay
Performed
Mon, Thu
Reported
1-8 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
 
Collect
Plain red or serum separator tube (SST) 
Specimen Preparation
Allow complete clotting of red blood cells (up to 1 hour), then separate serum from cells within 30 minutes and freeze immediately. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.3 mL) If ordered in conjunction with a Raji Cell Assay, transfer TWO (2) 1 mL aliquots of serum to individual ARUP Standard Transport Tubes. 
Storage/Transport Temperature
CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered. 
Unacceptable Conditions
Non-frozen specimens. 
Remarks
 
Stability
After separation from cells: Ambient: 2 hours; Refrigerated: Unacceptable; Frozen: 2 weeks (avoid repeated freeze/thaw cycles) 
Reference Interval
Less than 4 µgE/mL is considered negative for circulating complement binding immune complexes.
Interpretive Data
Circulating immune complexes may be found without any evident pathology and positive results do not necessarily implicate the immune complex in a disease process.

Note
CPT Code(s)
86332
Components
Component Test Code*Component Chart NameLOINC
0050301C1q Binding Assay27831-7
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • immune complex assay
  • Circulating complement binding immune complex
  • Circulating Immune Complex Detection
  • Immune Complex Assay