Borrelia burgdorferi Antibodies, IgG & IgM by Western Blot
0050254
Ordering Recommendation
Do not order in the absence of a positive/equivocal ELISA screening test. IgM Western blot is not useful after the first 4 weeks of clinical symptoms.
Mnemonic
LYME WB
Methodology
Qualitative Western Blot
Performed
Sun-Sat
Reported
1-2 days  
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Specimen Required
Patient Preparation
  
Collect
Serum separator tube.  
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.1 mL)  
Storage/Transport Temperature
Refrigerated.  
Unacceptable Conditions
CSF or plasma. Contaminated, heat-inactivated, hemolyzed, or severely lipemic specimens.  
Remarks
  
Stability
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)  
Reference Interval
Effective August 15, 2011

Negative  
Interpretive Data
IgG: For this assay, a positive result is reported when any 5 or more of the following 10 bands are present: 18, 23, 28, 30, 39, 41, 45, 58, 66, or 93 kDa. All other banding patterns are reported as negative.

IgM: For this assay, a positive result is reported when any 2 or more of the following bands are present: 23, 39, or 41 kDa. All other banding patterns are reported as negative.  
Note
Per CDC guidelines, if ELISA test result is NEGATIVE, Western blot should not be performed.

This test should be used for confirmation of an equivocal or positive Borrelia burgdorferi total antibodies, IgG and/or IgM test performed on patients less than four weeks after appearance of erythema migrans.
CPT Code(s)
86617 x2
Components
Component Test Code*Component Chart Name
0050252B. Burgdorferi, IgG WB
0050253B.Burgdorferi Antibody, IgM By WB
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Aliases
  • Lyme Antibodies
  • Lyme Disease
  • Lyme Disease Ab Western Blot
  • Lyme Disease Antibodies (Serum), WB
  • Lyme Disease antibodies WB