Borrelia burgdorferi Antibodies, IgG & IgM by Western Blot
Ordering Recommendation
Do not order in the absence of a positive/equivocal ELISA screening test. IgM Western blot is not useful after the first 4 weeks of clinical symptoms.
Qualitative Western Blot
1-2 days  
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Specimen Required
Patient Preparation
Serum separator tube.  
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.1 mL)  
Storage/Transport Temperature
Unacceptable Conditions
CSF or plasma. Contaminated, heat-inactivated, hemolyzed, or severely lipemic specimens.  
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)  
Reference Interval
Effective August 15, 2011

Interpretive Data
IgG: For this assay, a positive result is reported when any 5 or more of the following 10 bands are present: 18, 23, 28, 30, 39, 41, 45, 58, 66, or 93 kDa. All other banding patterns are reported as negative.

IgM: For this assay, a positive result is reported when any 2 or more of the following bands are present: 23, 39, or 41 kDa. All other banding patterns are reported as negative.  
Per CDC guidelines, if ELISA test result is NEGATIVE, Western blot should not be performed.

This test should be used for confirmation of an equivocal or positive Borrelia burgdorferi total antibodies, IgG and/or IgM test performed on patients less than four weeks after appearance of erythema migrans.
CPT Code(s)
86617 x2
Component Test Code*Component Chart NameLOINC
0050252B. Burgdorferi, IgG WB6320-6
0050253B.Burgdorferi Antibody, IgM By WB6321-4
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
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