Borrelia burgdorferi Antibody, IgM by Western Blot
0050253
Ordering Recommendation
Do not order in the absence of a positive/equivocal ELISA screening test. Useful only when ordered during first 4 weeks of clinical symptoms and concurrently with IgG Western blot test.
Mnemonic
LYME M WB
Methodology
Qualitative Western Blot
Performed
Sun-Sat
Reported
1-2 days  
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Specimen Required
Patient Preparation
  
Collect
Serum separator tube.  
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.1 mL)  
Storage/Transport Temperature
Refrigerated.  
Unacceptable Conditions
CSF or plasma. Contaminated, heat-inactivated, hemolyzed, or severely lipemic specimens.  
Remarks
  
Stability
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)  
Reference Interval
Effective August 15, 2011

Negative  
Interpretive Data
For this assay, a positive result is reported when any 2 or more of the following bands are present: 23, 39, or 41 kDa. All other banding patterns are reported as negative.  
Note
Current CDC recommendations for the serologic diagnosis of Lyme disease are to screen with a polyvalent EIA test and confirm equivocal and positive with Western Blot.  Both IgM and IgG Western blots should be performed on specimens less than four weeks after appearance of erythema migrans.  Only IgG Western blot should be performed on specimens greater than four weeks after disease onset. IgM Western blot in the chronic stage is not recommended and does not aid in the diagnosis of neuroborreliosis or chronic Lyme disease. Please submit requests for appropriate Western blot testing within 10 days.
CPT Code(s)
86617
Components
Component Test Code*Component Chart Name
0050253B.Burgdorferi Antibody, IgM By WB
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Aliases
  • Lyme Ab
  • Lyme Antibodies Detection
  • Lyme Antibodies IgM/IgG by Western Blot
  • Lyme Disease
  • Lyme Disease by PCR