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Borrelia burgdorferi Antibody, IgM by Immunoblot
0050253
Ordering Recommendation

Do not order in the absence of a positive/equivocal ELISA screening test. Useful only when ordered during first 4 weeks of clinical symptoms and concurrently with IgG immunoblot.

Mnemonic
LYME M WB
Methodology
Qualitative Immunoblot
Performed
Sun-Sat
Reported
1-2 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
 
Collect
Serum separator tube. 
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.15 mL) 
Storage/Transport Temperature
Refrigerated. 
Unacceptable Conditions
CSF or plasma. Contaminated, heat-inactivated, hemolyzed, or severely lipemic specimens. 
Remarks
 
Stability
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles) 
Reference Interval
Effective August 15, 2011

Negative
Interpretive Data
For this assay, a positive result is reported when any 2 or more of the following bands are present: 23, 39, or 41 kDa. All other banding patterns are reported as negative.

Note
Current CDC recommendations for the serologic diagnosis of Lyme disease are to screen with a polyvalent EIA test and confirm equivocal and positive with immunoblot.  Both IgM and IgG immunoblots should be performed on specimens less than 4 weeks after appearance of erythema migrans.  Only IgG immunoblot should be performed on specimens greater than 4 weeks after disease onset. IgM immunoblot in the chronic stage is not recommended and does not aid in the diagnosis of neuroborreliosis or chronic Lyme disease. Please submit requests for appropriate immunoblot testing within 10 days.
CPT Code(s)
86617
Components
Component Test Code*Component Chart NameLOINC
0050253B. burgdorferi Antibody IgM Immunoblot6321-4
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Lyme Ab
  • Lyme Antibodies Detection
  • Lyme Antibodies IgM/IgG by Western Blot
  • Lyme Disease
  • Lyme Disease by PCR