Borrelia burgdorferi Antibodies, Total by ELISA
Ordering Recommendation

First-line serologic screening test for suspected Lyme disease. Positive or equivocal results must be confirmed by immunoblot.

Semi-Quantitative Enzyme-Linked Immunosorbent Assay
1-2 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
Serum separator tube. 
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 2 mL serum to an ARUP Standard Transport Tube. (Min: 0.15 mL) 
Storage/Transport Temperature
Unacceptable Conditions
CSF. Contaminated, heat-inactivated, hemolyzed, or severely lipemic specimens. 
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles) 
Reference Interval
0.99 LIV or lessNegative - Antibody to B. burgdorferi not detected.
1.00-1.20 LIVEquivocal - Repeat testing in 10-14 days may be helpful.
1.21 LIV or greaterPositive - Probable presence of antibody to B. burgdorferi detected.

Interpretive Data

Once this test is performed, if:
a) Negative - no further testing is done;
b) Positive or equivocal - immunoblot testing will be performed on the original sample upon receiving a request. Sample will be held for 30 days only.

Current CDC recommendations for the serologic diagnosis of Lyme disease are to screen with a polyvalent ELISA test and confirm equivocal and positive results with Immunoblot.  Both IgM and IgG Immunoblots should be performed on specimens less than 4 weeks after appearance of erythema migrans.  Only IgG Immunoblot should be performed on specimens greater than 4 weeks after the disease onset.  IgM Immunoblot in the chronic stage is not recommended and does not aid in the diagnosis of neuroborreliosis or chronic Lyme disease.  Please submit requests for appropriate Immunoblot testing within 10 days.
Hotline History
Component Test Code*Component Chart NameLOINC
0050216Borrelia burgdorferi Abs, Total by ELISA5060-9
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • Lyme Antibodies Detection
  • Lyme Disease
  • Lyme Disease Ab ELISA