Borrelia burgdorferi Antibodies, Total by ELISA
0050216
Ordering Recommendation
First-line serologic screening test for suspected Lyme disease. Positive or equivocal results must be confirmed with a Western blot.
Mnemonic
LYME EIA
Methodology
Semi-Quantitative Enzyme-Linked Immunosorbent Assay
Performed
Sun, Tue-Sat
Reported
1-3 days  
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Specimen Required
Patient Preparation
  
Collect
Serum separator tube.  
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 2 mL serum to an ARUP Standard Transport Tube. (Min: 0.1 mL)  
Storage/Transport Temperature
Refrigerated.  
Unacceptable Conditions
CSF. Contaminated, heat-inactivated, hemolyzed, or severely lipemic specimens.  
Remarks
  
Stability
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)  
Reference Interval
 
 
These reference intervals are to be used in the interpretation of Borrelia burgdorferi Total Antibodies, IgG and/or IgM by ELISA result
0.99 LIV or less Negative -​ Antibody to Borrelia burgdorferi not detected.
1.00-​1.20 LIV Equivocal -​ Repeat testing in 10-​14 days may be helpful.
1.21 LIV or greater Positive -​ Probable presence of antibody to Borrelia burgdorferi detected.
Interpretive Data
Current CDC recommendations for the serologic diagnosis of Lyme disease are to screen with a polyvalent ELISA test and confirm equivocal and positive results with Western blot. Both IgM and IgG Western blots should be performed on specimens less than four weeks after appearance of erythema migrans. Only IgG Western blot should be performed on specimens greater than four weeks after the disease onset. IgM Western blot in the chronic stage is not recommended and does not aid in the diagnosis of neuroborreliosis or chronic Lyme disease. Please submit requests for appropriate Western blot testing within 10 days.  
Note
Once this test is performed, if:
a) Negative - no further testing is done;
b) Positive or equivocal - Western blot testing will be performed on the original sample upon receiving a request. Sample will be held for 30 days only.
CPT Code(s)
86618
Components
Component Test Code*Component Chart Name
0050216Borrelia burgdorferi Abs, Total by ELISA
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Aliases
  • Lyme Antibodies Detection
  • Lyme Disease
  • Lyme Disease Ab ELISA