Double-Stranded DNA (dsDNA) Antibody, IgG by ELISA with Reflex to dsDNA Antibody, IgG by IFA
0050215
Ordering Recommendation
Secondary screening for SLE based on ANA results.
Mnemonic
DNA
Methodology
Qualitative Enzyme-Linked Immunosorbent Assay/Semi-Quantitative Indirect Fluorescent Antibody
Performed
Sun-Sat
Reported
1-3 days  
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Specimen Required
Patient Preparation
  
Collect
Serum separator tube.  
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.5 mL)  
Storage/Transport Temperature
Refrigerated.  
Unacceptable Conditions
Plasma. Contaminated, hemolyzed, or severely lipemic specimens.  
Remarks
  
Stability
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen 1 year (avoid repeated freeze/thaw cycles)  
Reference Interval
Effective August 20, 2012
 
Test Number Components Reference Interval
 Double-​Stranded DNA (dsDNA) Antibody, IgG by ELISA None Detected.
2002693 Double-​Stranded DNA (dsDNA) Antibody, IgG by IFA (using Crithidia luciliae) Less than 1:10
Interpretive Data
Positivity for anti-double stranded DNA (anti-dsDNA) IgG antibody is a diagnostic criterion of systemic lupus erythematosus (SLE). Specimens are initially screened by enzyme-linked immunosorbent test (ELISA). All ELISA results reported as "detected" (positive) are confirmed by a highly specific IFA titer (Crithidia luciliae indirect fluorescent test [CLIFT]). Some patients with early or inactive SLE may be positive for anti-dsDNA IgG by ELISA but negative by CLIFT. If the patient is negative by CLIFT but positive by ELISA and clinical suspicion remains, consider antinuclear antibody (ANA) testing by IFA. Additional information and recommendations for testing may be found at http://www.arupconsult.com/Topics/AutoimmuneDz/ConnectiveTissueDz/index.html.  
Note
If dsDNA IgG antibody is detected, the result will be confirmed by Double-Stranded DNA (dsDNA) Antibody, IgG by IFA (using Crithidia luciliae). Additional charges apply.
CPT Code(s)
86225; if reflexed, add 86256
Components
Component Test Code*Component Chart NameLOINC
0050215Double-Stranded DNA (dsDNA) Ab IgG ELISA34187-5
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • anti-DNA
  • Anti-dsDNA Antibody
  • Anti-Native DNA
  • Antibody to ds-DNA
  • Crithidia luciliae
  • DNA ds Ab
  • Double stranded DNA antibody
  • ds DNA Ab
  • ds-DNA
  • ds-DNA IgG
  • dsDNA reflex
  • dsDNA reflex panel
  • Native double-stranded DNA antibody