Entamoeba histolytica (amebiasis), Antibody, IgG
Ordering Recommendation
Submit With Order
Semi-Quantitative Enzyme-Linked Immunosorbent Assay
Tue, Fri
1-5 days
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Serum separator tube.  
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.1 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as acute or convalescent.  
Storage/Transport Temperature
Unacceptable Conditions
Contaminated, heat-inactivated, hemolyzed, or severely lipemic specimens.  
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)  
Reference Interval
0.79 IV or less: Negative - No significant level of detectable E. histolytica IgG antibody.
0.80-1.19 IV: Equivocal - Repeat testing in 10-14 days may be helpful.
1.20 IV or greater: Positive - IgG antibody to E. histolytica detected, suggestive of a current or past infection.
Interpretive Data
Seroconversion between acute and convalescent sera is considered strong evidence of recent infection. The best evidence for infection is a significant change on two appropriately timed specimens where both tests are done in the same laboratory at the same time.
In the case of extraintestinal complications, a positive antibody can indicate amebiasis even though stool findings are negative.
CPT Code(s)
Component Test Code*Component Chart Name
0050070E. histolytica (amebiasis), Ab, IgG
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Cross References
  • Amebiasis histolytica Antibody
  • E histolytica IgG Antibody
  • E. histolytica Antibody
  • Ova and Parasite Exam