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Glomerular Basement Membrane Antibody, IgG (IFA)
0049191
Ordering Recommendation

May be useful in detecting glomerular basement membrane (GBM) antibodies. Combined use of IFA and multiplex bead assay (2008403) may improve diagnostic sensitivity for disease. If positive, may be useful for monitoring treatment response.

Mnemonic
GBM-G IFA
Methodology
Indirect Fluorescent Antibody
Performed
Mon-Fri
Reported
1-5 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
 
Collect
Serum separator tube.  
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transport 1 mL serum. (Min: 0.5 mL)  
Storage/Transport Temperature
Refrigerated.  
Unacceptable Conditions
 
Remarks
 
Stability
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)  
Reference Interval
Negative
Interpretive Data
When present, IgG antibody to glomerular basement membrane (GBM) antigen detected by either indirect fluorescent antibody (IFA) or multiplex bead assay helps support a diagnosis of Goodpasture syndrome. However, the combined result of both assays performed during initial evaluation improves the diagnostic sensitivity for disease. A positive result in one or both assays should be confirmed by renal biopsy.


Compliance Statement D: This test was developed and its performance characteristics determined by ARUP Laboratories. The U.S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.
Note
CPT Code(s)
86255
Components
Component Test Code*Component Chart NameLOINC
0049191GBM Antibody, IgG by IFA29994-1
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Anti-GBM Antibody
  • Anti-Glomerular Basement Membrane Antibody
  • GBM IgG IFA