Glomerular Basement Membrane Antibody, IgG (IFA)
0049191
Ordering Recommendation
 
Mnemonic
GBM-G IFA
Methodology
Indirect Fluorescent Antibody
Performed
Mon-Fri
Reported
Within 5 days  
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Specimen Required
Patient Preparation
  
Collect
Serum separator tube.  
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transport 1 mL serum. (Min: 0.5 mL)  
Storage/Transport Temperature
Refrigerated.  
Unacceptable Conditions
  
Remarks
  
Stability
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)  
Reference Interval
Negative  
Interpretive Data
The anti-GBM IFA methodology has been shown to be less specific than the semi-quantitative tests for circulating antibodies, such as the Glomerular Basement Membrane Antibody, IgG by Multiplex Bead Assay (ARUP Test Code 0051000). The antibody used in the Multiplex Bead Assay, although directed to human IgG F(ab')2, can bind to light chain sites common to all immunoglobulins, causing the assay to be more sensitive but less specific. The determination of a positive result is based on a diffuse "linear" staining pattern as viewed through a fluorescent microscope. All positive results should be confirmed by semi-quantitative tests and/or renal biopsy.

See Compliance Statement D: www.aruplab.com/CS
Statement D: This test was developed and its performance characteristics determined by ARUP Laboratories. The U.S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.
 
Note
 
CPT Code(s)
86255
Components
Component Test Code*Component Chart Name
0049191GBM Antibody, IgG by IFA
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Aliases
  • Anti-GBM Antibody
  • Anti-Glomerular Basement Membrane Antibody
  • GBM IgG IFA