Ordering Recommendation

May be useful in detecting glomerular basement membrane (GBM) antibodies. Combined use of IFA Glomerular Basement Membrane Antibody, IgG by Multiplex Bead Assay and IFA (2008403) may improve diagnostic sensitivity for disease. If positive, may be useful for monitoring treatment response.

New York DOH Approval Status

This test is New York state approved.

Specimen Required

Patient Preparation
Collect

Serum separator tube.

Specimen Preparation

Separate serum from cells ASAP or within 2 hours of collection. Transport 1 mL serum. (Min: 0.5 mL)

Storage/Transport Temperature

Refrigerated.

Unacceptable Conditions
Remarks
Stability

After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles)

Methodology

Indirect Fluorescent Antibody

Performed

Sun-Sat

Reported

1-3 days

Reference Interval

Negative

Interpretive Data

When present, IgG antibody to glomerular basement membrane (GBM) antigen detected by either indirect fluorescent antibody (IFA) or multiplex bead assay helps support a diagnosis of Goodpasture syndrome. However, the combined result of both assays performed during initial evaluation improves the diagnostic sensitivity for disease. A positive result in one or both assays should be confirmed by renal biopsy.

This test was developed and its performance characteristics determined by ARUP Laboratories. It has not been cleared or approved by the US Food and Drug Administration. This test was performed in a CLIA certified laboratory and is intended for clinical purposes.

Compliance Category

Laboratory Developed Test (LDT)

Note

Hotline History

N/A

CPT Codes

86255

Components

Component Test Code* Component Chart Name LOINC
0049191 GBM Antibody, IgG by IFA 29994-1
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.

Aliases

  • Anti-GBM Antibody
  • Anti-Glomerular Basement Membrane Antibody
  • GBM IgG IFA
Glomerular Basement Membrane Antibody, IgG (IFA)