ERBB2 (HER2/neu) (HercepTest) with Interpretation by Immunohistochemistry, Tissue
Ordering Recommendation

Aid in prediction of response to HER2-directed therapy in patients with breast carcinoma or gastroesophageal adenocarcinoma. Alternate test to confirm equivocal dual in situ hybridization (ISH) or fluorescence in situ hybridization (FISH) result. Refer to ARUP Immunohistochemistry Stain Offerings brochure at

1-5 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Immunohistochemistry Stain Form Recommended  (ARUP form #32978)Immunohistochemistry Stain Form Recommended (ARUP form #32978)
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
Tumor Tissue 
Specimen Preparation
Formalin fix (10 percent neutral buffered formalin) and paraffin embed tissue within 30 minutes of removal from patient. Fixative duration: 6-72 hours. Transport tissue block or 5 unstained (3-to 5-micron thick sections), positively charged slides in a tissue transport kit (ARUP supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800)522-2787. (Min: 3 slides) If sending precut slides, do not oven bake. 
Storage/Transport Temperature
Room temperature or refrigerated. Ship in cooled container during summer months. 
Unacceptable Conditions
Paraffin block with no tumor tissue remaining. Specimens with fixation delayed for more than 30 minutes. Specimens fixed in any other fixative other than 10 percent neutral buffered formalin. Tissue fixed for less than 6 hours or greater than 72 hours. Cytology specimens fixed in alcohol. 
Pathology report including tissue source and tumor origin must be submitted. Document time from tissue acquisition to fixation and fixation duration on requisition or enter at time of order. IMMUNOHISTOCHEMISTRY ORDERING AND SUBMISSION DETAILS: Submit electronic request. If you do not have electronic ordering capability, use an ARUP Immunohistochemistry form (#32978) with an ARUP client number. For additional technical details, please contact ARUP Client Services at (800) 522-2787. 
Ambient: Indefinitely; Refrigerated: Indefinitely ; Frozen: Unacceptable 
Reference Interval
Interpretive Data
Refer to report.

Hotline History
View Hotline History
Component Test Code*Component Chart NameLOINC
0049171Homogenous Pattern
0049184Intensity of Staining
0049188Percent of Cells/Circumferential
0049202ERBB2 (HercepTest) by IHC18474-7
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • c-erbB2 Hercept IHC
  • ERBB2 Hercept IHC
  • HER-2/neu Hercept IHC
  • HER2
  • HER2 IHC
  • Herceptin