Leukocyte Alkaline Phosphatase
Ordering Recommendation

Aid in differential diagnosis of neutrophilia, including chronic myeloid leukemia (CML) and leukemoid reaction.

Cytochemical Stain
1-5 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
  • Anatomic Pathology Test Request Form Recommended (ARUP form #32960)
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
Green (sodium or lithium heparin). 
Specimen Preparation
Protect both smears and whole blood from light and pack accordingly to avoid breakage. Transport 5 mL whole blood (Min: 1 mL) AND 6 unfixed, well-prepared smears (Min: 6 smears) 
Storage/Transport Temperature
Room temperature. Send Sunday through Thursday only. Whole blood must be received within 24 hours of collection; smears must be made within 24 hours of collection and received within 7 days of collection. 
Unacceptable Conditions
Specimens collected in EDTA. Poorly prepared smears (too thick or no feather edge). Broken or fixed smears.  Specimens that have been spun.  Smears made from anything other than heparin.  Smears made from blood older than 24 hours.  Whole blood not protected from light.  Smears not protected from light. 
Further information on how to make an adequate smear can be found in the following instructional video: 
 Blood: Ambient: 24 hours; Refrigerated: Unacceptable; Frozen: Unacceptable
Unfixed Smears:
Ambient: 1 week; Refrigerated: Unacceptable; Frozen: Unacceptable 
Reference Interval
Female: 33-149 (no units)
Male: 22-124 (no units)
Interpretive Data
Refer to Report

Component Test Code*Component Chart NameLOINC
0049000Leukocyte Alkaline Phosphatase4659-9
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • LAP