NPM1 Mutation by PCR and Fragment Analysis
0040174
Ordering Recommendation
Initial test for prognostication of CN-AML.
Submit With Order
Mnemonic
NPM1-F
Methodology
Polymerase Chain Reaction/Fragment Analysis
Performed
Varies
Reported
5-7 days
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
 
Collect
Lavender (EDTA) OR bone marrow (EDTA) OR tissue.  
Specimen Preparation
Whole blood: Transport 5 mL. (Min: 1 mL) OR Bone marrow: Transport 3 mL. (Min: 1 mL) OR FFPE tumor tissue: Formalin fix (10 percent neutral buffered formalin) and paraffin embed tissue. Protect from excessive heat. Transport tissue block or four 10-micron shavings. Tissue block will be returned after testing. Transport block(s) and/or shavings in a tissue transport kit (ARUP supply #47808) available online through eSupply using ARUP Connect™or contact ARUP Client Services at (800) 522-2787.  
Storage/Transport Temperature
Whole blood OR Bone marrow: Refrigerated. FFPE tumor tissue: Room temperature or refrigerated. Ship in cooled container during summer months.  
Unacceptable Conditions
Whole blood OR Bone marrow: Frozen specimens. Clotted or grossly hemolyzed specimens. FFPE tumor tissue: Specimens fixed/processed in alternative fixatives or heavy metal fixatives (B-4 or B-5) or tissue sections on slides. Decalcified specimens.  
Remarks
 
Stability
Ambient: 24 hours; Refrigerated: 5 days; Frozen: Unacceptable  
Reference Interval
Interpretive Data
Refer to report

See Compliance Statement B: www.aruplab.com/CS
Note
CPT Code(s)
81310
Components
Component Test Code*Component Chart Name
0040176NPM1 Mutation
2006956NPM1, Source
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Cross References
  • CN-AML prognostication
  • NPM1 exon 12