NPM1 Mutation by PCR and Fragment Analysis
0040174
Ordering Recommendation
Initial test for prognostication of CN-AML.
Mnemonic
NPM1-F
Methodology
Polymerase Chain Reaction/Fragment Analysis
Performed
Sun, Tue, Thu
Reported
5-7 days  
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Specimen Required
Patient Preparation
  
Collect
Lavender (EDTA) OR bone marrow (EDTA) OR tissue.  
Specimen Preparation
Whole blood: Transport 5 mL. (Min: 1 mL) OR Bone marrow: Transport 3 mL. (Min: 1 mL) OR FFPE tumor tissue: Formalin fix (10 percent neutral buffered formalin) and paraffin embed tissue. Protect from excessive heat. Transport tissue block or four 10-micron shavings. Tissue block will be returned after testing. Transport block(s) and/or shavings in a tissue transport kit (ARUP supply #47808) available online through eSupply using ARUP Connect™or contact ARUP Client Services at (800) 522-2787.  
Storage/Transport Temperature
Whole blood OR Bone marrow: Refrigerated. FFPE tumor tissue: Room temperature or refrigerated. Ship in cooled container during summer months.  
Unacceptable Conditions
Whole blood OR Bone marrow: Frozen specimens. Clotted or grossly hemolyzed specimens. FFPE tumor tissue: Specimens fixed/processed in alternative fixatives or heavy metal fixatives (B-4 or B-5) or tissue sections on slides. Decalcified specimens.  
Remarks
  
Stability
Ambient: 24 hours; Refrigerated: 5 days; Frozen: Unacceptable  
Reference Interval
   
Interpretive Data
Refer to report

See Compliance Statement B: www.aruplab.com/CS  
Note
 
CPT Code(s)
81310
Components
Component Test Code*Component Chart Name
0040176NPM1 Mutation
2006956NPM1, Source
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • CN-AML prognostication
  • NPM1 exon 12