KIT (D816V) Mutation by PCR
0040137
Ordering Recommendation
Aids in the diagnosis of mastocytosis. Provide prognostic and predictive information for tyrosine kinase inhibitor (TKI) therapy planning.
Mnemonic
KIT-F
Methodology
Polymerase Chain Reaction
Performed
DNA isolation: Sun-Sat
Assay:
Mon, Wed, Fri
Reported
2-7 days  
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Specimen Required
Patient Preparation
  
Collect
Lavender (EDTA) OR bone marrow (EDTA) OR tissue.  
Specimen Preparation
Whole blood: Transport 5 mL (Min: 1 mL) OR Bone marrow: Transport 3 mL (Min: 1 mL) OR Fresh tissue: Freeze immediately. Transport 100 mg or 0.5-2.0 cm3 tissue. OR FFPE tumor tissue: Formalin fix (10 percent neutral buffered formalin) and paraffin embed tissue. Protect from excessive heat. Transport tissue block or four 10-micron shavings. Tissue block will be returned after testing.  Transport tissue (frozen, paraffin blocks) in a tissue transport kit (ARUP supply #47808) available online through eSupply using ARUP Connect™or contact ARUP Client Services at (800) 522-2787.  
Storage/Transport Temperature
Whole blood OR Bone marrow: Refrigerated. Fresh tissue: Frozen on dry ice. FFPE tumor tissue: Room temperature or refrigerated. Ship in cooled container during summer months.  
Unacceptable Conditions
Whole blood OR Bone marrow: Frozen specimens. Clotted or grossly hemolyzed specimens. FFPE tumor tissue: Specimens fixed/processed in alternative fixatives or heavy metal fixatives (B-4 or B-5) or tissue sections on slides. Decalcified specimens.  
Remarks
  
Stability
Whole blood OR Bone marrow: Ambient: 24 hours; Refrigerated: 5 days; Frozen: Unacceptable
Fresh Tissue:
Ambient: Unacceptable; Refrigerated: 2 hours; Frozen: 1 year
FFPE tumor tissue:
Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable  
Reference Interval
   
Interpretive Data
Refer to report.

See Compliance Statement B: www.aruplab.com/CS
Statement B: This test was developed and its performance characteristics determined by ARUP Laboratories. The U.S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.
 
Note
 
CPT Code(s)
81402
Components
Component Test Code*Component Chart NameLOINC
0040133KIT (D816V) Mutation by PCR55201-8
2006959KIT, Source 
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Asp816Val
  • C-KIT
  • CKIT
  • D816V
  • KIT exon 17