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Kleihauer-Betke Stain for Fetal Hemoglobin
0040105
Ordering Recommendation
Mnemonic
KB
Methodology
Semi-Quantitative Acid Elution Eosin Stain/Microscopy
Performed
Sun-Sat
Reported
Within 24 hours
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
 
Collect
Lavender (EDTA) or pink (K2EDTA). 
Specimen Preparation
Transport 3 mL whole blood. (Min: 0.5 mL) 
Storage/Transport Temperature
Refrigerated. 
Unacceptable Conditions
Frozen specimens. Specimens collected after Rh immunoglobulin administered. Clotted specimens. 
Remarks
 
Stability
Ambient: 72 hours; Refrigerated: 72 hours; Frozen: Unacceptable 
Reference Interval
Negative
Interpretive Data
Volume (mL) of fetomaternal hemorrhage (assuming a maternal blood volume of 5 liters)=percent fetal rbcs x50.

The quantitation of fetomaternal hemorrhage by this method is inherently imprecise. Therefore, it is important to provide a safety margin when calculating the dose of RhIG.

Note
This test should be ordered on pregnant or post-partum women who need to be assessed for RhoGAM® or fetal-maternal bleeds. For routine fetal hemoglobin (Hb F) testing, please order Hemoglobin Evaluation with Reflex to Electrophoresis and/or HPLC (0050610).
CPT Code(s)
85460
Components
Component Test Code*Component Chart NameLOINC
0040105Kleihauer-Betke Stain4579-9
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Fetal hemoglobin stain
  • Fetal Hemoglobin, Semi-Quantitative
  • KB test
  • Kleihauer-Betke Stain