Kleihauer-Betke Stain for Fetal Hemoglobin
0040105
 
Ordering Recommendation
Mnemonic
KB
Methodology
Semi-Quantitative Acid Elution Eosin Stain/Microscopy
Performed
Sun-Sat
Reported
Within 24 hours
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
 
Collect
Lavender (EDTA) or pink (K2EDTA).  
Specimen Preparation
Transport 3 mL whole blood. (Min: 0.5 mL)  
Storage/Transport Temperature
Refrigerated.  
Unacceptable Conditions
Frozen specimens. Specimens collected after Rh immunoglobulin administered. Clotted specimens.  
Remarks
 
Stability
Ambient: 72 hours; Refrigerated: 72 hours; Frozen: Unacceptable  
Reference Interval
Negative
Interpretive Data
Volume (mL) of fetomaternal hemorrhage (assuming a maternal blood volume of 5 liters) = percent fetal rbcs x50.

The quantitation of fetomaternal hemorrhage by this method is inherently imprecise. Therefore, it is important to provide a safety margin when calculating the dose of RhIG.
Note
This test should be ordered on pregnant or post-partum women who need to be assessed for RhoGAM® or fetal-maternal bleeds. For routine fetal hemoglobin (Hb F) testing, please order Hemoglobin Evaluation with Reflex to Electrophoresis and/or HPLC (0050610).
CPT Code(s)
85460
Components
Component Test Code*Component Chart Name
0040105Kleihauer-Betke Stain
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, contact interface support at interface.support@aruplab.com.
Cross References
  • Fetal hemoglobin stain
  • Fetal Hemoglobin, Semi-Quantitative
  • KB test
  • Kleihauer-Betke Stain