Kleihauer-Betke Stain for Fetal Hemoglobin
0040105
Ordering Recommendation
 
Mnemonic
KB
Methodology
Semi-Quantitative Acid Elution Eosin Stain/Microscopy
Performed
Sun-Sat
Reported
Within 24 hours  
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Specimen Required
Patient Preparation
  
Collect
Lavender (EDTA) or pink (K2EDTA).  
Specimen Preparation
Transport 3 mL whole blood. (Min: 0.5 mL)  
Storage/Transport Temperature
Refrigerated.  
Unacceptable Conditions
Frozen specimens. Specimens collected after Rh immunoglobulin administered. Clotted specimens.  
Remarks
  
Stability
Ambient: 72 hours; Refrigerated: 72 hours; Frozen: Unacceptable  
Reference Interval
Negative  
Interpretive Data
Volume (mL) of fetomaternal hemorrhage (assuming a maternal blood volume of 5 liters)=percent fetal rbcs x50.

The quantitation of fetomaternal hemorrhage by this method is inherently imprecise. Therefore, it is important to provide a safety margin when calculating the dose of RhIG.  
Note
This test should be ordered on pregnant or post-partum women who need to be assessed for RhoGAM® or fetal-maternal bleeds. For routine fetal hemoglobin (Hb F) testing, please order Hemoglobin Evaluation with Reflex to Electrophoresis and/or HPLC (0050610).
CPT Code(s)
85460
Components
Component Test Code*Component Chart NameLOINC
0040105Kleihauer-Betke Stain4579-9
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Fetal hemoglobin stain
  • Fetal Hemoglobin, Semi-Quantitative
  • KB test
  • Kleihauer-Betke Stain