Recommended test to detect activated protein C resistance and confirm presence of a factor V Leiden mutation.
Clotting/Polymerase Chain Reaction/Fluorescence Monitoring
Refer to individual components
This test is New York DOH approved.
- Patient Preparation
- Lt. blue (sodium citrate) AND lavender (EDTA), pink (K2EDTA), or yellow (ACD Solution A or B). Refer to Specimen Handling at aruplab.com for hemostasis/thrombosis specimen handling guidelines.
- Specimen Preparation
- Transport 1.5 mL platelet-poor plasma AND 3 mL whole blood. (Min: 1 mL/each)
- Storage/Transport Temperature
- Plasma: Frozen. Whole Blood: Refrigerated.
- Unacceptable Conditions
- Plasma: Non-frozen specimens.
- Plasma: Ambient: 4 hours; Refrigerated: Unacceptable; Frozen at -20°C: 3 months; Frozen at -70°C: 6 months
Whole Blood: Ambient: 72 hours; Refrigerated: 1 week; Frozen: Unacceptable
|0030127||APC Resistance Profile
||2.0 or greater
|0097720||Factor V Leiden (F5) R506Q Mutation
||Negative: This sample is negative for factor V Leiden, R506Q mutation.
Ratios less than 2.00 suggest APC resistance. This method uses factor V deficient plasma; therefore, APC resistance due to a nonfactor V mutation will not be detected. Extreme factor V deficiency may cause abnormal ratio.
If APC resistance is normal, then no further testing will be added. If APC resistance is low, then Factor V Leiden by PCR will be added. Additional charges apply.
If reflexed, add 81241
|Component Test Code*||Component Chart Name|
|0030194||Factor V Leiden by PCR|
|2001308||FACV REF Specimen|
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
- Factor V Leiden Functional Assay with Reflex to PCR