APC Resistance Profile with Reflex to Factor V Leiden
Ordering Recommendation
Recommended test to detect activated protein C resistance and confirm presence of a factor V Leiden mutation.
Electromagnetic Mechanical Clot Detection/Polymerase Chain Reaction/Fluorescence Monitoring
1-5 days  
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Specimen Required
Patient Preparation
Lt. blue (sodium citrate) AND lavender (EDTA), pink (K2EDTA), or yellow (ACD Solution A or B). Refer to Specimen Handling at for hemostasis/thrombosis specimen handling guidelines.  
Specimen Preparation
Transport 1.5 mL platelet-poor plasma AND 3 mL whole blood. (Min: 1 mL/each)  
Storage/Transport Temperature
Plasma: CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered. Whole Blood: Refrigerated.  
Unacceptable Conditions
Serum, clotted or hemolyzed specimens.  
Plasma: Ambient: 4 hours; Refrigerated: Unacceptable; Frozen at -20°C: 3 months; Frozen at -70°C: 6 months
Whole Blood: Ambient: 72 hours; Refrigerated: 1 week; Frozen: Unacceptable  
Reference Interval
Test Number Components Reference Interval
0030127APC Resistance Profile 2.0 or greater
0097720Factor V Leiden (F5) R506Q Mutation Negative: This sample is negative for factor V Leiden, R506Q mutation.
Interpretive Data
Ratios less than 2.00 suggest APC resistance. This method uses factor V deficient plasma; therefore, APC resistance due to a nonfactor V mutation will not be detected. Extreme factor V deficiency may cause abnormal ratio.  
If APC resistance is normal, then no further testing will be added. If APC resistance is low, then Factor V Leiden by PCR will be added. Additional charges apply.
CPT Code(s)
85307; if reflexed, add 81241
If reflexed, add 81241
Component Test Code*Component Chart NameLOINC
0030193APC Resistance13590-5
0030194Factor V Leiden by PCR21668-9
2001308FACV REF Specimen 
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • Factor V Leiden Functional Assay with Reflex to PCR