APC Resistance Profile with Reflex to Factor V Leiden
Ordering Recommendation
Recommended test to detect activated protein C resistance and confirm presence of a factor V Leiden mutation.
Submit With Order
Clotting/Polymerase Chain Reaction/Fluorescence Monitoring
Refer to individual components
1-5 days
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Lt. blue (sodium citrate) AND lavender (EDTA), pink (K2EDTA), or yellow (ACD Solution A or B). Refer to Specimen Handling at aruplab.com for hemostasis/thrombosis specimen handling guidelines.  
Specimen Preparation
Transport 1.5 mL platelet-poor plasma AND 3 mL whole blood. (Min: 1 mL/each)  
Storage/Transport Temperature
Plasma: Frozen. Whole Blood: Refrigerated.  
Unacceptable Conditions
Plasma: Non-frozen specimens.  
Plasma: Ambient: 4 hours; Refrigerated: Unacceptable; Frozen at -20°C: 3 months; Frozen at -70°C: 6 months
Whole Blood: Ambient: 72 hours; Refrigerated: 1 week; Frozen: Unacceptable  
Reference Interval
Test Number Components Reference Interval
0030127APC Resistance Profile 2.0 or greater
0097720Factor V Leiden (F5) R506Q Mutation Negative: This sample is negative for factor V Leiden, R506Q mutation.
Interpretive Data
Ratios less than 2.00 suggest APC resistance. This method uses factor V deficient plasma; therefore, APC resistance due to a nonfactor V mutation will not be detected. Extreme factor V deficiency may cause abnormal ratio.
If APC resistance is normal, then no further testing will be added. If APC resistance is low, then Factor V Leiden by PCR will be added. Additional charges apply.
CPT Code(s)
If reflexed, add 81241
Component Test Code*Component Chart Name
0030193APC Resistance
0030194Factor V Leiden by PCR
2001308FACV REF Specimen
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Cross References
  • Factor V Leiden Functional Assay with Reflex to PCR