Thrombotic Risk, Inherited Etiologies (Most Common) with Reflex to Factor V Leiden
0030133
 
Ordering Recommendation
Acceptable panel to screen for common inherited thrombophilias.
Mnemonic
THROM COM
Methodology
Electromagnetic Clot Detection/Quantitative Enzymatic/Polymerase Chain Reaction/Fluorescence Monitoring
Performed
Sun-Sat
Reported
2-7 days
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Fasting preferred. Refer to Specimen Handling at aruplab.com for hemostasis/thrombosis specimen handling guidelines.  
Collect
Lt. blue (sodium citrate) AND lavender (EDTA) or pink (K2EDTA) AND serum separator tube or green (sodium or lithium heparin).  
Specimen Preparation
Serum Separator Tube or Green (sodium or lithium heparin) must be centrifuged and serum or plasma separated within 1 hour of collection. Transfer 1 mL centrifuged serum or plasma to ARUP Standard Transport Tube and label centrifuged tube for homocysteine testing. (Min: 0.5 mL) AND Transport two 2 mL aliquots platelet-poor plasma (sodium citrate) (Min: 1 mL/aliquot) AND Transport 5 mL whole blood (EDTA). (Min: 1 mL)  
Storage/Transport Temperature
Light blue (sodium citrate): CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered.
Lavender whole blood and Serum or Green (sodium or lithium heparin):
Refrigerated.  
Unacceptable Conditions
Specimens collected in any tube type not listed above.  
Remarks
 
Stability
Light blue (sodium citrate): Ambient: Unacceptable; Refrigerated: Unacceptable; Frozen: 2 weeks
Lavender whole blood:
Ambient: 2 hours; Refrigerated: 1 week; Frozen: Unacceptable
Serum or Green (sodium or lithium heparin):
Ambient: 1 hour; Refrigerated: 1 week; Frozen: 3 months  
Reference Interval
Effective November 18, 2013
Test Number Components Reference Interval
0030235Partial Thromboplastin Time 24-​35 seconds
0030095Factor VIII, Activity 0-​6 years: 56-​191%
7-​9 years: 76-​199%
10-​11 years: 80-​209%
12-​13 years: 72-​198%
14-​15 years: 69-​237%
16-​17 years: 63-​221%
18 years and older: 56-​191%
0099869Homocysteine, Total Less than 11 µmol/L, for both male and female
0056060Prothrombin (F2) G20210A Mutation Negative: This sample is negative for the factor II, prothrombin 20210A mutation.
0030127APC Resistance Profile 2.0 or greater
Factor V Leiden by PCR & Fluorescence Monitoring Negative: The sample is negative for factor V Leiden, R506Q mutation.
Interpretive Data
Ratios less than 2.00 suggest APC resistance. This method uses factor V deficient plasma; therefore, APC resistance due to a nonfactor V mutation will not be detected. Extreme factor V deficiency may cause abnormal ratio. Elevated total homocysteine (tHcy) concentrations may be associated with vitamin B12 deficiency, folate deficiency, or inherited disorders of methionine metabolism. tHcy may also be used as a weak-graded risk factor for cardiovascular disease or stroke.
Note
If APC resistance is normal, then no further testing will be added. If APC resistance is abnormally low, then Factor V Leiden by PCR will be added. Additional charges apply. False elevations of plasma or serum homocysteine may occur if the plasma or serum is not promptly separated from the cells at the time of collection.
CPT Code(s)
85730, 85240, 83090, 81240, 85307; If reflexed, add 81241
Components
Component Test Code*Component Chart Name
0030052Thrombosis Interpretation
0030095Factor VIII, Activity
0030193APC Resistance
0030194Factor V Leiden by PCR
0030235Partial Thromboplastin Time
0056060Prothrombin (F2) G20210A Mutation
0099869Homocysteine, Total
2001308FACV REF Specimen
2001334PT PCR Specimen
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, contact interface support at interface.support@aruplab.com.
Cross References
  • Activated Protein C Resistance
  • APC Resistance
  • APCR
  • F2 G20210A mutation
  • F8 Activity
  • Factor V Leiden
  • Factor V Leiden Functional Assay
  • Factor V Leiden PCR
  • Factor VIII, Activity
  • FVL
  • Hypercoag
  • Hypercoagulable Panel
  • Protein C Resistance
  • Prothrombin mutation
  • prothrombin time
  • PTT
  • total homocyteine