ADAMTS13 Activity
0030056
Ordering Recommendation
Submit With Order
Mnemonic
ADAMTS-13
Methodology
Fluorescence Resonance Energy Transfer
Performed
Mon-Fri
Reported
1-4 days
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
 
Collect
Lt. blue (sodium citrate). Refer to Specimen Handling at aruplab.com for hemostasis/thrombosis specimen handling guidelines.  
Specimen Preparation
Transfer 1 mL platelet-poor plasma to an ARUP Standard Transport Tube. (Min: 0.5 mL)  
Storage/Transport Temperature
CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered.  
Unacceptable Conditions
Hemolyzed specimens. Serum or EDTA plasma.  
Remarks
 
Stability
Ambient: 3 hours; Refrigerated: Unacceptable; Frozen: 2 weeks  
Reference Interval
Greater than or equal to 67 percent.
Interpretive Data
Microtiter plates coated with monoclonal anti-ADAMTS13 antibody are used to measure ADAMTS13 activity by Fluorescence Resonance Energy Transfer (FRET).

ADAMTS13 levels of less than 5% may be associated with either inherited (Upshaw-Schulman Syndrome) or acquired thrombotic thrombocytopenic purpura (TTP).

A variety of medical conditions may result in a mild to moderate deficiency of ADAMTS13 activity. Recent plasma exchange therapy may raise the observed ADAMTS13 activity.

The monoclonal anti-ADAMTS13 antibody is directed against the CUB domain; therefore, certain forms of ADAMTS13 (with mutations in the CUB domains) may not be equivalently measured due to reduced binding to the antibody.

See Compliance Statement D: www.aruplab.com/CS
Note
CPT Code(s)
85397
Components
Component Test Code*Component Chart Name
0030049ADAMTS13 Activity
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Cross References
  • von Willebrand Factor Cleaving Protease