ADAMTS13 Activity
0030056
Ordering Recommendation
 
Mnemonic
ADAMTS-13
Methodology
Fluorescence Resonance Energy Transfer
Performed
Mon-Fri
Reported
1-4 days  
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Specimen Required
Patient Preparation
  
Collect
Lt. blue (sodium citrate). Refer to Specimen Handling at aruplab.com for hemostasis/thrombosis specimen handling guidelines.  
Specimen Preparation
Transfer 1 mL platelet-poor plasma to an ARUP Standard Transport Tube. (Min: 0.5 mL)  
Storage/Transport Temperature
CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered.  
Unacceptable Conditions
Hemolyzed specimens. Serum or EDTA plasma.  
Remarks
  
Stability
Ambient: 3 hours; Refrigerated: Unacceptable; Frozen: 2 weeks  
Reference Interval
Greater than or equal to 67 percent.  
Interpretive Data
Microtiter plates coated with monoclonal anti-ADAMTS13 antibody are used to measure ADAMTS13 activity by Fluorescence Resonance Energy Transfer (FRET).

ADAMTS13 levels of less than 5% may be associated with either inherited (Upshaw-Schulman Syndrome) or acquired thrombotic thrombocytopenic purpura (TTP).

A variety of medical conditions may result in a mild to moderate deficiency of ADAMTS13 activity. Recent plasma exchange therapy may raise the observed ADAMTS13 activity.

The monoclonal anti-ADAMTS13 antibody is directed against the CUB domain; therefore, certain forms of ADAMTS13 (with mutations in the CUB domains) may not be equivalently measured due to reduced binding to the antibody.

See Compliance Statement D: www.aruplab.com/CS  
Note
 
CPT Code(s)
85397
Components
Component Test Code*Component Chart Name
0030049ADAMTS13 Activity
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • von Willebrand Factor Cleaving Protease