Human Immunodeficiency Virus 1 RNA Quantitative bDNA
0020466
 
Ordering Recommendation
Mnemonic
HIV BDNA
Methodology
Quantitative Branched Chain DNA
Performed
Tue, Fri
Reported
3-8 days
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
 
Collect
Lavender (EDTA), pink (K2EDTA), or plasma preparation tube.  
Specimen Preparation
Separate EDTA plasma from cells within 4 hours. Transfer 3 mL plasma to an ARUP Standard Transport Tube. (Min: 1.5 mL)
PPT:
Separate plasma from cells within 2 hours. Transport 3 mL plasma in original container. (Min: 1.5 mL)  
Storage/Transport Temperature
Frozen.  
Unacceptable Conditions
Serum. Heparinized specimens.  
Remarks
 
Stability
PPT: On Cells: Ambient: 2 hours; After separation from cells: Ambient: 24 hours; Refrigerated: Unacceptable; Frozen at -20°C: 72 hours; Frozen at -70°C: 4 months
All others:
On Cells: Ambient: 4 hours; After separation from cells: Refrigerated: 48 hours; Frozen at -20°C: 72 hours; Frozen at -70°C: 4 months  
Reference Interval
Not Detected
Interpretive Data
The quantitative range of this assay is 1.9-5.7 log copies/mL (75-500,000 copies/mL).

An interpretation of "Not Detected" does not rule out the presence of inhibitors in the patient specimen or HIV-1 virus RNA concentrations below the level of detection of the assay. Care should be taken in the interpretation of any single viral load determination. The clinical significance of changes in HIV-1 RNA measurements has not been fully established; however, a change of 0.5 log copies/mL may be significant. Low positive values may occasionally be seen in specimens from patients who are uninfected.

This assay should not be used for blood donor screening, associated re-entry protocols, or for screening Human Cell, Tissues and Cellular Tissue-Based Products (HCT/P).
Note
The test is intended for use in conjunction with clinical presentation and other laboratory markers as an indicator of disease prognosis. This test is also used as an aid in assessing viral response to antiretroviral treatment as measured by changes in HIV-1 RNA levels.
CPT Code(s)
87536
Components
Component Test Code*Component Chart Name
0020464HIV-1 RNA Quant bDNA, Log
0051816HIV-1 RNA Quant bDNA Interpretation
2002687HIV-1 RNA Quant bDNA, Copy
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, contact interface support at interface.support@aruplab.com.
Cross References
  • HIV 1 branched DNA
  • HIV 1 NAAT
  • HIV 1 Nucleic Acid Amplification Test
  • HIV 1 viral load monitoring
  • HIV 1RNA bDNA quantitative
  • HIV quantitative
  • HIV-1 RNA
  • Signal Amplification