Iron and Iron Binding Capacity
Ordering Recommendation
Aids in the diagnosis of iron deficiency, anemia, and iron overload.
Submit With Order
Quantitative Spectrophotometry
Within 24 hours
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
Serum separator tube.  
Specimen Preparation
Allow specimen to clot completely at room temperature. Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.5 mL) Avoid hemolysis. Also acceptable: Heparinized plasma.  
Storage/Transport Temperature
Unacceptable Conditions
Grossly hemolyzed specimens. EDTA plasma.  
After separation from cells: Ambient: 1 week; Refrigerated: 1 week; Frozen: 3 months  
Reference Interval
Test Number Components Reference Interval
Iron, Plasma or Serum Newborn 0-​6 weeks: 100-​250 µg/dL
Infant 7 weeks -​ 11 months: 40-​100 µg/dL
Child 1 year -​ 10 years: 50-​120 µg/dL
Male 11 years and older: 50-​170 µg/dL
Female 11 years and older: 30-​160 µg/dL
Transferrin Saturation 20-​50%
Total Iron Binding CapacityUIBC: Not reported on patient reports:
0-​2 months: 59-​175 µg/dL
3 months-​17 years: 250-​400 µg/dL
18 years and older: 240-​450 µg/dL
Interpretive Data
CPT Code(s)
83540; 83550
Component Test Code*Component Chart Name
0020037Iron, Serum or Plasma
0020049Iron Binding Capacity Total
0020050Transferrin Saturation
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Cross References
  • Fe
  • Fe and TIBC
  • IBC
  • Iron
  • IRON AND TIBC, Serum
  • Iron Binding Capacity
  • Iron Indices
  • Latent Iron Binding Capacity
  • Serum Iron and TIBC Routine
  • Serum Iron-Binding Capacity
  • TIBC
  • Total Iron-Binding Capacity
  • Transferrin Saturation
  • Transferrin Saturation Percent