Lymphocyte Proliferation, Antigen-Mitogen Panel by Flow Cytometry (24-Hr Critical Room Temp)
3001319
Ordering Recommendation

Primarily for evaluating lymphocyte function in patients with suspected cellular immune dysfunction, such as primary and secondary immunodeficiencies. Other uses include monitoring lymphocyte recovery and competence after hematopoietic stem cell transplantation and monitoring lymphocyte function during immunosuppressive therapy. Do not order for patients younger than 3 months unless clinical history of candidiasis is present.

Mnemonic
LAMP FLOW
Methodology
Cell Culture/Flow Cytometry
Performed
Thu, Fri
Reported
9-10 days
New York DOH Approval Status
Specimens from New York clients will be sent out to a New York DOH approved laboratory, if possible.
Submit With Order
Time SensitiveTime Sensitive
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
Collect control specimen from a healthy individual unrelated to patient.
Patient and control specimens must be collected ONLY on Wednesdays or Thursdays AND shipped directly to ARUP the same calendar day to meet the strict 24-hour stability requirement. 
Collect
Green (sodium heparin) (patient) AND green (sodium heparin) (control) only on Wednesdays or Thursdays. 
Specimen Preparation
Transport 10 mL whole blood (patient) AND 10 mL whole blood (control) in original collection tubes. (Min: 7 mL (patient) AND 7 mL (control)). Infant Minimum: 3 mL (patient) AND 7 mL (control).
Do not refrigerate or freeze. LIVE LYMPHOCYTES REQUIRED. 
Storage/Transport Temperature
CRITICAL ROOM TEMPERATURE.
Must be collected and shipped directly to ARUP the same calendar day.
 
Unacceptable Conditions
Refrigerated or frozen specimens. 
Remarks
Do not collect or ship on, or the day before, holidays. 
Stability
Ambient: 24 hours; Refrigerated: Unacceptable; Frozen: Unacceptable 
Reference Interval
PHACon APWM
CD45 Pos Cells39.7% or greater15.5% or greater10.0% or greater
CD3 Pos Cells42.5% or greater17.4% or greater8.0% or greater
CD19 Pos Cells51.9% or greaterN/A6.3% or greater
TetanusCandida
CD45 Pos Cells2.9% or greater9.6% or greater
CD3 Pos Cells3.0% or greater2.8% or greater

Interpretive Data
This test measures lymphocyte proliferation in response to stimulation with nonspecific mitogens phytohemagglutinin (PHA), concanavalin A (Con A), and pokeweed mitogen (PWM); and T lymphocyte proliferation in response to stimulation with recall antigens tetanus toxoid and Candida, determined by flow cytometry. Proliferating cells are detected by fluorescent labeling. Results are reported as percent proliferating cells of total specific cell populations.

Statement B: Compliance Statement B: For laboratory developed tests not using a RUO kit, and for FDA approved, cleared or 510(k) exempt assays with alterations. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.

Note
Hotline History
View Hotline History
Components
Component Test Code*Component Chart NameLOINC
3001322CD45+ Cell Proliferation by PHA69038-8
3001323CD3+ Cell Proliferation by PHA69013-1
3001324CD19+ Cell Proliferation by PHA
3001325CD45+ Cell Proliferation by Con A
3001326CD3+ Cell Proliferation by Con A
3001327CD45+ Cell Proliferation by PWM69019-8
3001328CD3+ Cell Proliferation by PWM69020-6
3001329CD19+ Cell Proliferation by PWM69037-0
3001330CD45+ Cell Proliferation by Tetanus69016-4
3001331CD3+ Cell Proliferation by Tetanus69029-7
3001332CD45+ Cell Proliferation by Candida69014-9
3001333CD3+ Cell Proliferation by Candida69015-6
3001334Lymphocyte Antigen Mitogen Panel Interp53810-8
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please view this test within the Laboratory Test Directory found at www.aruplab.com
Aliases