Antinuclear Antibody (ANA) with HEp-2 Substrate, IgG by IFA
3000082
Ordering Recommendation

Preferred ANA screening test for connective tissue disease.

Mnemonic
ANA IFA AB
Methodology
Semi-Quantitative Indirect Fluorescent Antibody
Performed
Sun-Sat
Reported
1-3 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
 
Collect
Serum Separator Tube (SST). 
Specimen Preparation
Separate from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.15 mL) 
Storage/Transport Temperature
Refrigerated. 
Unacceptable Conditions
Plasma. Contaminated, hemolyzed, or severely lipemic specimens. 
Remarks
 
Stability
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles) 
Reference Interval
Less than 1:80
Interpretive Data
Presence of antinuclear antibodies (ANA) is a hallmark feature of systemic autoimmune rheumatic diseases (SARD). ANA lacks diagnostic specificity and is associated with a variety of diseases (cancers, autoimmune, infectious, and inflammatory conditions) and may also occur in healthy individuals in varying prevalence. The lack of diagnostic specificity requires confirmation of positive ANA by more-specific serologic tests. Diagnosis may be aided by the pattern(s) observed.

Negative results do not necessarily rule out SARD.

Note
ANA are determined by indirect fluorescence assay (IFA) using HEp-2 substrate and IgG-specific conjugate at a screening dilution of 1:80. If positive, patterns reported include homogeneous, speckled, centromere, nucleolar, nuclear dots, or cytoplasmic. All positive results are reported with endpoint titers, at no additional charge.
Hotline History
View Hotline History
Components
Component Test Code*Component Chart NameLOINC
3000090Antinuclear Antibody (ANA), HEp-2, IgG21424-7
3000096ANA Interpretive Comment49311-4
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please view this test within the Laboratory Test Directory found at www.aruplab.com
Aliases
  • ANA
  • Antinuclear Antibodies
  • Antinuclear Antibody
  • FANA
  • Fluorescent Antinuclear Antibodies