Monitor engraftment of maternal T cells in patients with severe combined immunodeficiency (SCID) prior to allogenic stem cell transplantation.
- Patient Preparation
- Lavender (EDTA), Pink (K2EDTA), or Yellow (ACD Solution A).
- Specimen Preparation
- Transport 3 mL whole blood. (Min: 3 mL) Increase the amount of blood submitted for patients with low cell counts.
- Storage/Transport Temperature
- Room temperature. Ship overnight. Specimens should be received within 24 hours of collection for optimal isolation of T cells.
- Unacceptable Conditions
- Clotted or hemolyzed specimens.
- Please provide the results and date of the patient's most recent WBC and differential counts.
- Ambient: 48 hours; Refrigerated: 48 hours; Frozen: Unacceptable
|Type Maternal||Maternal cells only.|
|Type Patient||Patient cells only.|
|Mixed||Patient and Maternalt cells present. Semi-quantitative results of percentage of patient and maternal cells will be reported.|
Indication: Severe combined immunodeficiency (SCID) patients lack T cells and cannot recognize and reject maternal T cells from maternal-fetal transfusion. Maternal T cell can proliferate in the absence of host T cells, leading to difficulty in determining the host T cell numbers required for the diagnosis of SCID and/or can cause graft-versus-host disease-line (GVHD) presentation.
Methodology: PCR followed by capillary electrophoresis. Specimens are analyzed using 15 autosomal markers (D8S1179, D21S11, D7S820, CSF1PO, D3S1358, THO1, D13S317, D16S539, D2S1338, D19S433, vWa, TPOX, D18S51, D5S818, and FGA) and one gender marker (amelogenin).
Kit Used: AmpFLSTR Identifiler PCR Amplification Kit, Applied Biosystems.
Limit of Detection: 2 percent of minor cell population.
|Component Test Code*||Component Chart Name||LOINC|
|2014700||Specimen, Maternal Engraftment|
|2014701||Mat Engraftment, Informative Loci|
|2014702||Maternal Engraftment, Interpretation|
- Maternal engraftment
- SCID engraftment