Detect respiratory pathogens in patients with pneumonia.
- Patient Preparation
- Bronchoalveolar lavage (BAL)
- Specimen Preparation
- Transfer 2 mL to a sterile container. (Min: 1.2 mL)
- Storage/Transport Temperature
- Unacceptable Conditions
- Specimen source required.
- Ambient: Unacceptable; Refrigerated: 4 days; Frozen: 30 days
Negative results do not rule out viral, bacterial, or fungal infections. Targeted, PCR-based tests are generally more sensitive and are preferred when specific pathogens are suspected, especially for DNA viruses (Adenovirus, CMV, HHV6, HSV, and VZV), mycobacteria, and fungi.
The analytical sensitivity of this test depends on the cellularity of the sample and the concentration of all microbes present. Analytical sensitivity is assessed using Internal Controls that are added to each sample. Sequencing data for Internal Controls is quantified. Samples with Internal Control values below the validated minimum may have reduced analytical sensitivity or contain inhibitors and are reported as 'Reduced Analytical Sensitivity'. Additional respiratory pathogens to those reported cannot be excluded in samples with 'Reduced Analytical Sensitivity'.
Due to the complexity of next generation sequencing methodologies, there may be a risk of false-positive results. Contamination with organisms from the upper respiratory tract during specimen collection can also occur. The detection of viral, bacterial, and fungal nucleic acid does not imply organisms causing invasive infection. Results from this test need to be interpreted in conjunction with the clinical history, results of other laboratory tests, epidemiologic information, and other available data. Confirmation of positive results by an alternate method may be indicated in select cases.
Test developed and characteristics determined by ARUP Laboratories.
|Component Test Code*||Component Chart Name||LOINC|
|2013695||Respiratory Pathogens by NGS||48767-8|
|2013696||EER Respiratory Pathogens by NGS||11526-1|
|2013697||Respiratory Pathogens by NGS, Source||31208-2|