Cortisol, Free by Equilibrium Dialysis/LC-MS/MS
Ordering Recommendation

Screen, diagnose, and monitor diseases associated with excess or deficient cortisol production.

Equilibrium Dialysis/Quantitative High Performance Liquid Chromatography-Tandem Mass Spectrometry
Sun, Tue, Thu, Fri
2-5 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
Recommended collection times are 8-10 a.m. or 4-6 p.m.  
Plain red or serum separator tube, lavender (EDTA) or pink (K2EDTA). 
Specimen Preparation
Separate serum or plasma from cells within 2 hours of collection. Transfer 1 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 0.6 mL) 
Storage/Transport Temperature
Unacceptable Conditions
Grossly hemolyzed, icteric, lipemic or heparinized specimens.  
Indicate time of draw on test request form and specimen tube. 
After separation from cells: Ambient: Unacceptable; Refrigerated: 1 week; Frozen: 6 months 
Reference Interval
AgeTime of CollectionReference Range
0 - 17 years Not established
18 years of age and older8-10 a.m. collection0.21 - 1.04 µg/dL
18 years of age and older4-6 p.m. collection0.10 - 0.63 µg/dL

Interpretive Data

Statement B: Compliance Statement B: For laboratory developed tests not using a RUO kit, and for FDA approved, cleared or 510(k) exempt assays with alterations. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.

To convert to nmol/L, multiply µg/dL by 27.6.
Hotline History
Component Test Code*Component Chart NameLOINC
2012698Cortisol, Free by ED/LC-MS/MS2145-1
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please view this test within the Laboratory Test Directory found at
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