SSA 52 and 60 (Ro) (ENA) Antibodies, IgG
2012074
Ordering Recommendation

Order as secondary screen based on results of ANA test or if ANA IFA is negative and Sj√∂gren syndrome, systemic lupus erythematosus (SLE), systemic sclerosis, or myositis is strongly suspected.

Mnemonic
SSA RO
Methodology
Semi-Quantitative Multiplex Bead Assay
Performed
Sun-Sat
Reported
1-2 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
 
Collect
Serum separator tube (SST). 
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.2 mL) 
Storage/Transport Temperature
Refrigerated. 
Unacceptable Conditions
Plasma or other body fluids. Contaminated, hemolyzed, or severely lipemic specimens. 
Remarks
 
Stability
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles) 
Reference Interval
Test Number
Components
Reference Interval
SSA-52 (Ro52) (ENA) Antibody, IgG29 AU/mL or Less: Negative
30-40 AU/mL: Equivocal
41 AU/mL or greater: Positive
SSA-60 (Ro60) (ENA) Antibody, IgG29 AU/mL or Less: Negative
30-40 AU/mL: Equivocal
41 AU/mL or greater: Positive

Interpretive Data
SSA-52 (Ro52) and/or SSA-60 (Ro60) antibodies are associated with a diagnosis of Sjögren syndrome, systemic lupus erythematosus (SLE), and systemic sclerosis. SSA-52 antibody overlaps significantly with the major SSc-related antibodies. SSA-52 (Ro52) antibody occurs frequently in patients with inflammatory myopathies, often in the presence of interstitial lung disease.

Note
Hotline History
N/A
Components
Component Test Code*Component Chart NameLOINC
0050691SSA-52 (Ro52) (ENA) Antibody, IgG31625-7
2012055SSA-60 (Ro60) (ENA) Antibody, IgG53019-6
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please view this test within the Laboratory Test Directory found at www.aruplab.com
Aliases
  • Anti-Ro
  • Anti-SSA; Anti-SSA (Ro) (ENA) Antibody
  • Ro
  • Ro Autoantibodies
  • Sjogren Syndrome
  • Sjogren's Antibody
  • SSA Autoantibodies