ALK (D5F3) by Immunohistochemistry with Reflex to ALK Gene Rearrangements by FISH
Ordering Recommendation

Detects ALK fusion proteins (IHC) and ALK gene rearrangements (FISH) in solid tumors.

Immunohistochemistry/Fluorescence in situ Hybridization
1-5 days; 12 days if reflexed
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
Tumor tissue. 
Specimen Preparation
Formalin fix (10 percent neutral buffered formalin) and paraffin embed specimen. Protect paraffin block and/or slides from excessive heat. Transport tissue block or 7 unstained (4-micron thick sections), positively charged slides in a tissue transport kit (ARUP supply #47808 recommended but not required) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787. (Min. 4 slides). If sending precut slides, do not oven bake. 
Storage/Transport Temperature
Room temperature. Also acceptable: Refrigerated. Ship in cooled container during summer months. 
Unacceptable Conditions
Paraffin block with no tumor tissue remaining; specimens fixed in any fixative other than 10 percent neutral buffered formalin. 
Include surgical pathology report. 
Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable. 
Reference Interval
Interpretive Data
Refer to report

Note: This test includes pathologist interpretation of results. Include surgical pathology report with specimen submitted for testing. Indicate tissue site on requisition. ALK (D5F3) by Immunohistochemistry will reflex to FISH if result is Equivocal or Positive. Additional charges apply.
Hotline History
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CPT Code(s)
Component Test Code*Component Chart NameLOINC
2007325ALK(D5F3) by IHC Reference Number57723-9
2007327ALK(D5F3) by IHC Result35474-6
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please view this test within the Laboratory Test Directory found at
  • ALK D5F3 antibody staining
  • ALK D5F3 staining
  • ALK rearrangement by IHC
  • ALK rearrangement staining