Helicobacter pylori Breath Test, Pediatric
Ordering Recommendation

Use to diagnose H. pylori infection in children and adolescents (3 to 17 years of age). For adults, refer to Helicobacter pylori Breath Test, Adult (2010476).

Qualitative Spectrophotometry
1-4 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
This test requires the pediatric patient (3-17 years old) to fast for 1 hour prior to test administration. The patient should not have taken antibiotics, proton pump inhibitors (e.g., Prilosec, Prevacid, Aciphex, Nexium), or bismuth preparations (e.g., Pepto-Bismol) within the previous 14 days. The effect of histamine 2-receptor antogonists (e.g., Axid, Pepcid, Tagamet, Zantac) may reduce urease activity on urea breath tests and should be discontinued for 24-48 hours before the sample is collected. When used to monitor treatment, the test should be performed four weeks after cessation of definitive therapy. The patient should be informed that the Pranactin-Citric drink that will be administered contains phenylalanine. Phenylketonurics restrict dietary phenylalanine. 
BreathTek UBT Kit. (ARUP Supply #51124) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787. 
Specimen Preparation
1) Label breath collection bags with patient name, MRN, date and time of collection, and indicate Pre (blue) or Post (pink).
2) Collect the baseline (Pre) breath specimen according to the instructions in the BreathTek UBT kit.
3) After the allotted time, collect the Post breath specimen according to the instructions in the kit. 
Storage/Transport Temperature
Unacceptable Conditions
Underinflated bags. Specimens from patients younger than 3 years. 
Submit with Order: Weight (in pounds), height (in inches), sex, and age. 
Ambient: 1 week; Refrigerated: Unacceptable; Frozen: Unacceptable 
Reference Interval
Interpretive Data
A negative result does not rule out the possibility of H. pylori infection. If clinical signs are suggestive of H. pylori infection, retest with a new specimen or an alternate method. Known causes of false-negative results include:
1. Use of antibiotics, proton pump inhibitors, and bismuth preparations during the preceding 2 weeks.
2. Administration of the breath test less than 4 weeks after completion of definitive therapy to eradicate H. pylori.
3. Premature or late collection of the post-dose specimen.

Known causes of false-positive results include:
1. Patients with achlorhydria.
2. Rinsing the testing solution in the mouth or not using the straw provided in the kit, which can allow contact with urease-positive bacteria.
3. The presence of other gastric spiral organisms such as Helicobacter heilmanii.

The post-dose sample must be collected between 15 and 30 minutes post-dose to prevent a false- negative result.
Hotline History
View Hotline History
Component Test Code*Component Chart NameLOINC
0020646H. pylori Breath Test29891-9
2010926Patient height at time of collection
2010927Patient weight at time of collection
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please view this test within the Laboratory Test Directory found at www.aruplab.com
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