• Identify eosinophil involvement by staining for eosinophil granule major basic protein 1 (eMBP1) and eosinophil-derived neurotoxin (EDN) to detect both cellular localization and extracellular deposition.
• Eosinophil granule proteins are toxic to tissues and cells and may be pathogenic in disease but not recognized when deposited outside of morphologically identifiable intact eosinophils.
- Patient Preparation
- Specimen Preparation
- Transport tissue (optimal 3-8 mm) in Michel's medium (ARUP supply #45462) available online through eSupply using ARUP Connect or call ARUP Client Services at (800) 522-2787. (Min: 1 mm).
Also acceptable: Zeus tissue fixative, flash frozen fresh tissue, formalin fixed tissue, or formalin fixed and paraffin embedded tissue. Transport in formalin, tissue block, or slides with two 5-micron sections per slide in serial order and numbered. (Min: 8 slides).
- Storage/Transport Temperature
- Room temperature.
Flash Frozen Fresh Tissue: Frozen
- Unacceptable Conditions
- Michel's Medium or Zues Tissue Fixative: Ambient: 10 days; Refrigerated: 10 days; Frozen: Unacceptable
Formalin Fixed Tissue: Ambient: 3 weeks; Refrigerated: 3 weeks; Frozen: Unacceptable
Flash Frozen Fresh Tissue: Ambient: Unacceptable; Refrigerated: Unacceptable; Frozen: Indefinitely
Paraffin Embedded Tissue: Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Indefinitely
|Component Test Code*||Component Chart Name||LOINC|
|2010911||EER Eosinophil Granule MBP in Tissues||11526-1|
|2010922||Eosinophil Granule MBP in Tissues||10478-6|