MYD88 L265P Mutation Detection by PCR, Quantitative
2009318
Ordering Recommendation
Useful in distinguishing lymphoplasmacytic lymphoma (LPL) from other low-grade B-cell lymphoproliferative disorders which may be in the differential diagnosis. Use when monitoring patients with LPL diagnosis and previously identified MYD88 L265P mutation.
Mnemonic
MYD88
Methodology
Real-time Polymerase Chain Reaction
Performed
DNA isolation: Sun-Sat
Testing: Varies
Testing: Varies
Reported
5-10 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
- Patient Preparation
- Collect
- Lavender (EDTA), bone marrow (EDTA), or FFPE tumor tissue. Also acceptable: DNA extracted by CLIA certified lab.
- Specimen Preparation
- Whole Blood: Do not freeze. Transport 5 mL whole blood. (Min: 1 mL)
Bone Marrow: Do not freeze. Transport 3 mL bone marrow. (Min: 1 mL)
FFPE Tumor Tissue: Formalin fix (10 percent neutral buffered formalin) and paraffin embed tissue. Protect from excessive heat. Transport tissue in a tissue transport kit (ARUP Supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.
Extracted DNA: Transport 40 uL DNA with at least 50 ng/uL concentration. (Min: 40 uL) Transport DNA in a tissue transport kit (ARUP Supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787. - Storage/Transport Temperature
- Whole Blood, Bone Marrow or Extracted DNA: Refrigerated.
FFPE Tumor Tissue: Room temperature. Also acceptable: Refrigerated. Ship in cooled container during summer months. - Unacceptable Conditions
- Whole Blood, Bone Marrow or Extracted DNA: Plasma, serum, or DNA extracted by a non-CLIA lab. Specimens collected in anticoagulants other than EDTA or sodium heparin. Clotted or grossly hemolyzed specimens.
FFPE Tumor Tissue: Specimens fixed/processed in alternative fixatives, heavy metal fixatives (B-4 or B-5), or tissue sections on slides. Decalcified specimens. - Remarks
- Stability
- Whole Blood or Bone Marrow: Ambient: 24 hours; Refrigerated: 5 days; Frozen: Unacceptable
FFPE Tumor Tissue: Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable
Extracted DNA: Ambient: 1 month; Refrigerated: Indefinitely; Frozen: Indefinitely
Reference Interval
Interpretive Data
Refer to report.
Statement B: Compliance Statement B: For laboratory developed tests not using a RUO kit, and for FDA approved, cleared or 510(k) exempt assays with alterations. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.
Note
Hotline History
View Hotline History
CPT Code(s)
Components
| Component Test Code* | Component Chart Name | LOINC |
|---|---|---|
| 2009319 | MYD88 Source | |
| 2009320 | MYD88 L265P Detection, Result | |
| 2009321 | MYD88 L265P Detection, Quant |
Aliases
- Lymphoplasmacytic lymphoma
- Waldenstrom macroglobulinemia