MGMT Promoter Methylation Detection
2009310
Ordering Recommendation

Aid in treatment decisions in individuals with high-grade gliomas.

Mnemonic
MGMT
Methodology
Polymerase Chain Reaction/MassARRAY/MALDI-TOF
Performed
DNA isolation: Sun-Sat
Assay: Varies
Reported
8-12 days
New York DOH Approval Status
Specimens from New York clients will be sent out to a New York DOH approved laboratory, if possible.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
 
Collect
Tumor tissue. Also acceptable: DNA extracted by CLIA certified lab with corresponding client-circled H&E slide. 
Specimen Preparation
Tumor Tissue: Formalin fix (10 percent neutral buffered formalin) and paraffin embed tissue. Protect from excessive heat. Tissue block will be returned after testing. Transport tissue block or 5 unstained 5-micron slides. (Min: 3 slides) Transport block and/or slide(s) in a tissue transport kit (ARUP Supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.
Extracted DNA: Transport 40 uL DNA with at least 50 ng/uL concentration. (Min: 40 uL) Transport DNA in a tissue transport kit (ARUP Supply #47808) available online through eSupply using ARUP ConnectTM or contact ARUP Client Services at (800) 522-2787. 
Storage/Transport Temperature
Room temperature. Ship in cooled container during summer months.
Extracted DNA: Refrigerated. 
Unacceptable Conditions
Specimens fixed/processed in alternative fixatives (alcohol, Prefer) or heavy metal fixatives (B-4 or B-5). Decalcified specimens. Less than 25 percent tumor. 
Remarks
Include surgical pathology report. 
Stability
Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable
Extracted DNA: Ambient: 1 month; Refrigerated: Indefinitely; Frozen: Indefinitely 
Reference Interval
Interpretive Data
Refer to report.

Statement B: Compliance Statement B: For laboratory developed tests not using a RUO kit, and for FDA approved, cleared or 510(k) exempt assays with alterations. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.

Note
Hotline History
View Hotline History
Components
Component Test Code*Component Chart NameLOINC
2002148Block ID57723-9
2009311MGMT Methylation Detection - Results60252-4
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please view this test within the Laboratory Test Directory found at www.aruplab.com
Aliases