Paraneoplastic Antibodies (PCCA/ANNA) by IFA with Reflex to Titer and Immunoblot
Ordering Recommendation

Aid in the diagnosis of paraneoplastic neurologic syndromes associated with malignancy. Order based on clinical presentation.

Semi-Quantitative Indirect Fluorescent Antibody/Qualitative Immunoblot
Sun, Wed, Fri
1-6 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
Serum separator tube 
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 2 mL serum to an ARUP Standard Transport Tube. (Min: 0.75 mL) 
Storage/Transport Temperature
Unacceptable Conditions
Contaminated, heat-inactivated, hemolyzed, or lipemic specimens. 
After separation from cells: Ambient: 24 hours; Refrigerated: 2 weeks; Frozen: 1 year 
Reference Interval
Test Number
Reference Interval
Purkinje Cell/Neuronal Nuclear IgG ScrnNone Detected
Neuronal Nuclear Antibody (ANNA) IFA Titer, IgGLess than 1:10
Purkinje Cell Antibody, TiterLess than 1:10
2007963Neuronal Nuclear Antibodies (Hu, Ri, Yo) IgG by ImmunoblotNone Detected

Interpretive Data

Statement D: Compliance Statement D: For laboratory tests using a manufactured RUO kit. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.

Purkinje Cell (PCCA) antibody and Neuronal Nuclear (ANNA) antibody IgG are screened by IFA. If the IFA screen is indeterminate, then a Neuronal Nuclear Antibodies (Hu, Ri, and Yo) IgG by Immunoblot will be added. If the IFA screen is positive at 1:10 or greater, then a PCCA/ANNA antibodies titer and Neuronal Nuclear Antibodies (Hu, Ri, and Yo) IgG by Immunoblot will be added. Additional charges apply.
Hotline History
CPT Code(s)
86255; if reflexed add 83516 and/or 86256
Component Test Code*Component Chart NameLOINC
2007962Purkinje Cell/Neuronal Nuclear IgG Scrn61177-2
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please view this test within the Laboratory Test Directory found at
  • ANNA
  • ANNA-1
  • ANNA-2
  • Anti Ri
  • Anti-Hu
  • Anti-Yo
  • Antinuclear neuronal Ab
  • Neuronal Nuclear Antibody
  • Paraneoplastic Ab screen
  • Paraneoplastic Autoantibody Immunoblot Confirmation, Paraneoplastic Neurological Autoimmunity
  • PCA-1
  • Purkinje Cell Cytoplasmic Antibodies