Celiac Disease Dual Antigen Screen with Reflex
Ordering Recommendation

Acceptable reflexive screening test for celiac disease. Positive screen results reflex to IgA and IgG antibody testing for tissue transglutaminase antibody (tTG) and deaminated gliadin peptide antibody (DGP). Preferred screening test is Celiac Disease Reflexive Cascade (2008114) or Tissue Transglutaminase (tTG) Antibody, IgA (0097709) testing.

Semi-Quantitative Enzyme-Linked Immunosorbent Assay
Mon, Wed, Fri
1-6 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
Serum separator tube. 
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 2 mL serum to an ARUP Standard Transport Tube. (Min: 1 mL) 
Storage/Transport Temperature
Unacceptable Conditions
Plasma. Contaminated, hemolyzed, or grossly lipemic specimens. 
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year 
Reference Interval

Test Number
Reference Interval
0051689Celiac Disease Dual Antigen Screen
19 Units or lessNegative - No significant level of detectable IgA or IgG antibodies against human tissue transglutaminase or gliadin peptide.
20 Units or greaterPositive - Presence of IgA and/or IgA antibodies against human tissue transglutaminase and/or gliadin peptide; suggests possibility of certain gluten sensitive enteropathies such as celiac disease and dermatitis herpetiformis.

0097709Tissue Transglutaminase (tTG) Antibody, IgA
3 U/mL or lessNegative
4-10 U/mLWeak Positive
11 U/mL or greaterPositive

0056009Tissue Transglutaminase Antibody, IgG
5 U/mL or lessNegative
6-9 U/mLWeak Positive
10 U/mL or greaterPositive

0051357Deamidated Gliadin Peptide (DGP) Antibody, IgA
19 Units or lessNegative
20-30 UnitsWeak Positive
31 Units or greaterPositive

0051359Deamidated Gliadin Peptide (DGP) Antibody, IgG
19 Units or lessNegative
20-30 UnitsWeak Positive
31 Units or greaterPositive

Interpretive Data
Refer to individual components.

The Celiac Disease Dual Antigen Screen is a combined antibody assay for tissue transglutaminase and deamidated gliadin-derived peptide antibodies, IgA and IgG.

If Celiac Disease Dual Antigen Screen is 20 Units or greater, then tTG IgA and Gliadin Peptide IgA will be added. If tTG IgA and Gliadin Peptide IgA are negative, tTG IgG and Gliadin Peptide IgG will be added. Additional charges apply.
Hotline History
CPT Code(s)
83516; if reflexed, add 83516 x2; if further reflexed, add 83516 x2
Component Test Code*Component Chart NameLOINC
0051691Celiac Disease Dual Antigen Screen63420-4
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please view this test within the Laboratory Test Directory found at www.aruplab.com
  • Anti-tissue Transglutaminase Antibody
  • CD Dual Ag screen
  • Celiac Sprue
  • Deaminated Gliadin Peptide Antibodies
  • DGP
  • DGP IgA IgG
  • Dual Ag reflex screen
  • Dual antigen screen, Dual Ag screen
  • Gluten Sensitivity Screen
  • Gluten-Sensitive Enteropathy Tests
  • tTG IgG, IgA