Bordetella pertussis Antibody, IgM by ELISA with Reflex to Immunoblot
Ordering Recommendation

Evaluation of IgM pertussis antibodies has little clinical utility. CDC-recommended first-line tests for pertussis are Bordetella pertussis/parapertussis by PCR (0065080) and/or Bordetella pertussis Culture (0060117).

Semi-Quantitative Enzyme-Linked Immunosorbent Assay/Qualitative Immunoblot
1-8 days
New York DOH Approval Status
Specimens from New York clients will be sent out to a New York DOH approved laboratory, if possible.
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
Serum separator tube. 
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.1 mL) Parallel testing is preferred and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as "acute" or "convalescent." 
Storage/Transport Temperature
Unacceptable Conditions
Contaminated, heat-inactivated, or severely lipemic specimens. 
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles) 
Reference Interval
Test Number
Reference Interval
Bordetella pertussis Antibody, IgM by ELISAEffective February 20, 2018
0.9 IV or less: Negative - No significant level of detectable B. pertussis IgM antibody.
1.0-1.1 IV: Equivocal - Repeat testing in 10-14 days may be helpful.
1.2 IV or greater: Positive - IgM antibody to B. pertussis detected, which may indicate a current or recent exposure/immunization to B. pertussis.
2004326Bordetella pertussis Antibody, IgM by Immunoblot
Bordetella pertussis Antibody, IgM by Immunoblot
Effective February 19, 2013
ComponentsReference Interval
Bordetella pertussis Ab, IgM by Immunoblot InterpNegative
B. pertussis, IgM Immunoblot PTNegative
B. pertussis, IgM Immunoblot FHANegative

Interpretive Data
Recommend that treatment decisions be based on the result of the B. pertussis IgM immunoblot test instead of the ELISA test. B. pertussis IgM test by ELISA may produce false-positive results.

Statement A: Compliance Statement A: For laboratory developed tests using a manufacturer labeled ASR as the reagent providing the specificity of the assay. Analyte Specific Reagent. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, the FDA has determined that such clearance or approval is not necessary.

If Bordetella pertussis Antibody, IgM by ELISA is 1.2 IV or greater, then Bordetella pertussis IgM Immunoblot testing will be added. Additional charges apply.
Hotline History
View Hotline History
CPT Code(s)
Component Test Code*Component Chart NameLOINC
2001783B. pertussis Ab, IgM by ELISA42329-3
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please view this test within the Laboratory Test Directory found at
  • B. pertussis IgM reflex
  • Pertussis Antibody
  • Pertussis IgM Ab ELISA reflex to immunoblot