Cutaneous Direct Immunofluorescence, Biopsy
Ordering Recommendation

• Order concurrently with serum antibody testing and fixed tissue histopathology for assessment of patient with pruritic, urticarial, blistering and/or erosive disorders, including possible pemphigoid and pemphigoid variants, pemphigus and pemphigus subtypes, dermatitis herpetiformis, epidermolysis bullous acquisita, porphyria, and pseudoporphyria.
• Order concurrently with fixed tissue histopathology for assessment of patient with inflammatory, immune-mediated cutaneous disease, including possible lupus and lupus variants, vasculitis, drug reactions, lichen planus and lichenoid reactions.

Direct Immunofluorescence
3-7 days
New York DOH Approval Status
This test is New York DOH approved.
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
Tissue: skin, mucosa (oral, conjunctival, genital, esophageal), other epithelium (gastrointestinal, respiratory, urinary). 
Specimen Preparation
Transport tissue (optimal 4-6 mm) in Michel's medium (ARUP supply #45462) available online through eSupply using ARUP Connect or call ARUP Client Services at (800) 522-2787. Also acceptable: Zeus tissue fixative. 
Storage/Transport Temperature
Room temperature. Also acceptable: Refrigerated. 
Unacceptable Conditions
Formalin-fixed tissue. Solid organs or solid organ tissue. 
Ambient: 10 days; Refrigerated: 10 days; Frozen: Unacceptable 
Reference Interval
By report
Interpretive Data

Hotline History
View Hotline History
Component Test Code*Component Chart NameLOINC
0092571Cutaneous Direct IF, Biopsy6471-7
2010916EER Cutaneous Direct IF, Biopsy
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please view this test within the Laboratory Test Directory found at
  • Bullous Disease
  • Chronic Bullous Disease
  • Cutaneous Herpetiformis
  • Dermatitis Herpetiformis
  • Lichen Planus and Lichenoid
  • Linear IgA Bullous, Tissue
  • Lupus Erythematous, Tissue
  • Pemphigoid, Tissue
  • Pemphigus
  • Porphyria and Pseudoporphyria, Tissue
  • Skin Immunofluorescence
  • Urticaria
  • Urticarial Pemphigoid
  • Urticarial Vasculitis
  • Vasculitis