Testosterone, Females or Children
Ordering Recommendation

Use in conjunction with free testosterone in the evaluation of suspected hyperandrogenemia in women and children.

Quantitative High Performance Liquid Chromatography-Tandem Mass Spectrometry
1-4 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
Collect between 6-10 a.m. 
Serum separator tube or green (sodium or lithium heparin). 
Specimen Preparation
Separate serum or plasma from cells ASAP or within 2 hours of collection. Transfer 1 mL serum or plasma to an ARUP Standard Transport Tube. (Min: 0.2 mL) 
Storage/Transport Temperature
Unacceptable Conditions
EDTA plasma. 
This test is suggested for women and children due to an improved sensitivity of testosterone by LC-MS/MS. 
After separation from cells: Ambient: 24 hours; Refrigerated: 1 week; Frozen: 6 months 
Reference Interval
Effective August 19, 2013
Premature (26-28 weeks)5-16 ng/dL59-125 ng/dL
Premature (31-35 weeks)5-22 ng/dL37-198 ng/dL
Newborn20-64 ng/dL75-400 ng/dL
1-5 monthsLess than 20 ng/dL14-363 ng/dL
6-24 monthsLess than 9 ng/dLLess than 37 ng/dL
2-3 yearsLess than 20 ng/dLLess than 15 ng/dL
4-5 yearsLess than 30 ng/dLLess than 19 ng/dL
6-7 yearsLess than 7 ng/dLLess than 13 ng/dL
8-9 years1-11ng/dL2-8 ng/dL
10-11 years3-32 ng/dL2-165 ng/dL
12-13 years6-50 ng/dL3-619 ng/dL
14-15 years6-52 ng/dL31-733 ng/dL
16-17 years9-58 ng/dL158-826 ng/dL
18-39 years9-55 ng/dL300-1080 ng/dL
40-59 years9-55 ng/dL300-890 ng/dL
60 years and older5-32 ng/dL300-720 ng/dL
Premenopausal (18 years and older)9-55 ng/dLDoes Not Apply
Postmenopausal5-32 ng/dLDoes Not Apply
Tanner Stage I2-17 ng/dL2-15 ng/dL
Tanner Stage II5-40 ng/dL3-303 ng/dL
Tanner Stage III10-63 ng/dL10-851 ng/dL
Tanner Stage IV-V11-62 ng/dL162-847 ng/dL

Interpretive Data
Total testosterone values may not reflect optimal concentrations in all individuals. Free or bioavailable testosterone measurements may provide supportive information.

Statement B: Compliance Statement B: For laboratory developed tests not using a RUO kit, and for FDA approved, cleared or 510(k) exempt assays with alterations. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.

Hotline History
Component Test Code*Component Chart NameLOINC
0081058Testosterone, LC-MS/MS2986-8
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please view this test within the Laboratory Test Directory found at www.aruplab.com
  • Total Testosterone