Acid-Fast Bacillus (AFB) Identification with Reflex to Susceptibility
Ordering Recommendation

Order for M. tuberculosis complex speciation and susceptibility testing.

Identification/Susceptibility. Methods may include biochemical, mass spectrometry, nucleic acid probe, polymerase chain reaction, or sequencing.
Varies (depends on organism growth)
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
Actively growing organism, in pure culture. 
Specimen Preparation
Transport sealed container with pure culture on solid or in liquid media. Place each specimen in an individually sealed bag. 
Storage/Transport Temperature
Room temperature. If culture is suspected of being a microorganism identified on the IATA list as an infectious substance affecting humans, submit specimen according to Infectious Substance, Category A, shipping guidelines. 
Unacceptable Conditions
Mixed cultures or non-viable organisms. Organisms submitted on agar plate. 
Indicate source of organism and any other pertinent information such as growth rate of the organism or DNA probe results. 
Ambient: 2 weeks; Refrigerated: 2 weeks; Frozen: 2 weeks 
Reference Interval
Complete identification and susceptibility of clinically significant isolates of M. tuberculosis complex, M. kansasii, M. avium-intracellulare complex, M. fortuitum complex, M. abscessus complex, M. chelonae, M. immunogenum and any isolate from a significant source.
Interpretive Data
For identification by DNA sequencing and MALDI-TOF:
See Compliance Statement B:

If a significant organism listed above is identified, then the appropriate susceptibility panel will be added. Additional charges apply. For other organisms, contact ARUP if susceptibility testing is clinically indicated.

A processing fee will be billed for all organisms submitted that are not in pure culture as requested in the specimen requirements. Identification of AFB isolates is billed by individual tests performed.
Hotline History
CPT Code(s)
CPT codes for identification and susceptibility vary based on method.
Component Test Code*Component Chart NameLOINC
0060997AFB Identification/Susceptibility29579-0
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please view this test within the Laboratory Test Directory found at
  • ID by sequencing
  • M. tuberculosis identification
  • MA AFB
  • Mycobacteria identification
  • Mycobacterium tuberculosis identification
  • Primary Anti-tuberculosis agents
  • Primary Panel