Herpes Simplex Virus by PCR
Ordering Recommendation

Preferred test for detecting herpes simplex virus (HSV) infection in CSF, neonates, or when rapid diagnostic test for suspected HSV infection is necessary.

Qualitative Polymerase Chain Reaction
1-3 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
Lavender (EDTA), pink (K2EDTA), or serum separator tube. OR Amniotic fluid, bronchoalveolar lavage (BAL), CSF, ocular fluid, tissue, vesicle fluid. OR Endocervical specimen in ThinPrep® Pap Test media. 
Specimen Preparation
Separate plasma or serum from cells. Transfer 1 mL serum, plasma, amniotic fluid, BAL, CSF, ocular fluid, or ThinPrep specimen to a sterile container. (Min: 0.5 mL) 
Transfer to a sterile container and freeze immediately. 
Vesicle Fluid:
Transfer to viral transport media (ARUP supply #12884). Available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787. 
Storage/Transport Temperature
Unacceptable Conditions
Heparinized specimens. 
Specimen source required. 
Tissue: Ambient: Unacceptable; Refrigerated: Unacceptable; Frozen: 3 months
All Others: Ambient: 8 hours; Refrigerated: 72 hours; Frozen: 3 months 
Reference Interval
Interpretive Data

Statement B: Compliance Statement B: For laboratory developed tests not using a RUO kit, and for FDA approved, cleared or 510(k) exempt assays with alterations. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.

Hotline History
Component Test Code*Component Chart NameLOINC
0050102Herpes Simplex Virus Source31208-2
0060041Herpes Simplex Virus by PCR5014-6
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please view this test within the Laboratory Test Directory found at www.aruplab.com
  • Herpes Simplex Virus, Type 1/2 DNA, Real-Time PCR,
  • HSV
  • HSV Types 1 and 2