APC Resistance Profile with Reflex to Factor V Leiden
Ordering Recommendation

Recommended test to detect activated protein C resistance and confirm presence of a factor V Leiden variant.

Electromagnetic Mechanical Clot Detection/Polymerase Chain Reaction/Fluorescence Monitoring
1-5 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
Light Blue (Sodium Citrate) AND Lavender (EDTA), Pink (K2EDTA), or Yellow (ACD Solution A or B). Refer to Specimen Handling at aruplab.com for hemostasis/thrombosis specimen handling guidelines. 
Specimen Preparation
Transport 1.5 mL platelet-poor plasma AND 3 mL whole blood. (Min: 1 mL/each) 
Storage/Transport Temperature
Plasma: CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered.
Whole Blood:
Unacceptable Conditions
Serum, clotted or hemolyzed specimens. 
Plasma: Ambient: 4 hours; Refrigerated: Unacceptable; Frozen at -20°C: 3 months; Frozen at -70°C: 6 months
Whole Blood: Ambient: 72 hours; Refrigerated: 1 week; Frozen: Unacceptable 
Reference Interval
Test Number
Reference Interval
0030127APC Resistance Profile2.0 or greater
0097720Factor V Leiden (F5) R506Q MutationNegative: This sample is negative for factor V Leiden, R506Q mutation.

Interpretive Data
Ratios less than 2.00 suggest APC resistance. This method uses factor V deficient plasma; therefore, APC resistance due to a nonfactor V mutation will not be detected. Extreme factor V deficiency may cause abnormal ratio.

If APC resistance is normal, then no further testing will be added. If APC resistance is low, then Factor V Leiden by PCR will be added. Additional charges apply.
Hotline History
View Hotline History
CPT Code(s)
Component Test Code*Component Chart NameLOINC
0030193APC Resistance13590-5
0030194Factor V Leiden by PCR21668-9
2001308FACV REF Specimen31208-2
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please view this test within the Laboratory Test Directory found at www.aruplab.com
  • Activated Protein C Resistance
  • APCR
  • Factor V Leiden Functional Assay with Reflex to PCR
  • Protein C Resistance