Hepatitis B Virus Surface Antigen with Reflex to Confirmation
Ordering Recommendation

Can be ordered as part of the acute hepatitis panel which includes HAV IgM, HBV core antibody IgM, HBV surface antigen, and HCV antibody. Refer to Hepatitis Panel, Acute with Reflex to HBsAg Confirmation (0020457).

Qualitative Chemiluminescent Immunoassay  
Within 24 hours
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
Serum separator tube. 
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 2 mL serum to an ARUP Standard Transport Tube. (Min: 1 mL) Also acceptable: K2EDTA plasma. 
Storage/Transport Temperature
Unacceptable Conditions
Heparinized plasma. Specimens containing particulate material. Heat-inactivated, severely hemolyzed or lipemic specimens. 
After separation from cells: Ambient: Unacceptable; Refrigerated: 1 week; Frozen: Indefinitely (avoid repeated freeze/thaw cycles) 
Reference Interval
Test Number
Reference Interval
Hepatitis B Virus Surface AntigenNegative
0020128Hepatitis B Virus Surface Antigen, ConfirmationNon Confirmed

Interpretive Data
This panel of assays should not be used for blood donor screening, associated re-entry protocols, or for screening Human Cell, Tissues and Cellular and Tissue-Based Products (HCT/P).

Performed and Reported times indicated are for screening of the HBsAg. If results for HBsAg screen are repeatedly reactive with an index value between 1.00 and 50.00, then HBsAg Confirmation will be added. Additional charges apply.
Hotline History
View Hotline History
CPT Code(s)
Component Test Code*Component Chart NameLOINC
0020089Hepatitis B Surface Antigen5196-1
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please view this test within the Laboratory Test Directory found at www.aruplab.com
  • Chronic Hep Screen B
  • HBsAg
  • HBsAg screen
  • HBV Surface Antigen
  • Hep B Surface Antigen
  • Hepatitis Bs Ag